Laser-capture microdissection and transcriptional profiling of the dorsomedial nucleus of the hypothalamus

Department of Internal Medicine and Department of Pharmacology, Division of Hypothalamic Research, University of Texas Southwestern Medical Center, Dallas, Texas 75390-9077.
The Journal of Comparative Neurology (Impact Factor: 3.23). 11/2012; 520(16):3617-32. DOI: 10.1002/cne.23116
Source: PubMed

ABSTRACT Identifying neuronal molecular markers with restricted patterns of expression is a crucial step in dissecting the numerous pathways and functions of the brain. While the dorsomedial nucleus of the hypothalamus (DMH) has been implicated in a host of physiological processes, current functional studies have been limited by the lack of molecular markers specific for DMH. Identification of such markers would facilitate the development of mouse models with DMH-specific genetic manipulations. Here we used a combination of laser-capture microdissection (LCM) and gene expression profiling to identify genes that are highly expressed within the DMH relative to adjacent hypothalamic regions. Six of the most highly expressed of these genes, Gpr50, 4930511J11Rik, Pcsk5, Grp, Sulf1, and Rorβ, were further characterized by real-time polymerase chain reaction (PCR) analysis and in situ hybridization histochemistry. The genes identified in this article will provide the basis for future gene-targeted approaches for studying DMH function. J. Comp. Neurol. 520:3617-3632, 2012. © 2012 Wiley Periodicals, Inc.

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    • "Both VM and DM are heterogeneous entities composed of different cell types whose identity and spatial distribution have not been fully resolved yet. Various chemo-and genoarchitectonic mappings suggest in both cases a fundamental organization into compact core portions and surrounding dispersed shell domains (Milhouse 1973; Chou et al. 2001; Choi et al. 2005; Segal et al. 2005; McClellan et al. 2006; Lee et al. 2012; Puelles et al. 2012). The latter authors noted that the VM and DM core domains are nearly completely excitatory (glutamatergic), whereas their shell regions, VMs, DMs, contain more dispersed inhibitory GABAergic neurons intermixed with glutamatergic ones (Puelles et al. 2012; their Figs. "
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    • "Endocannabinoids and NO that are co-released from DMH neurons differentially regulate GABAergic inhibitory tone and fasting reinforces NO-mediated enhancement of GABAergic currents [14]. Although a recent study further identifies genes that are highly expressed in the DMH using microarray analysis [15], little information is available about molecular markers specific for the DMH, which would facilitate the development of mouse models with DMH-specific genetic manipulations. "
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