Article

A microplate technique to simultaneously assay calcium accumulation in endoplasmic reticulum and SERCA release of inorganic phosphate

Department of Neurology, Uniformed Services University of the Health Sciences, B-3059, 4301 Jones Bridge Road, Bethesda, MD 20814, USA. .
Biological Procedures Online (Impact Factor: 1.3). 04/2012; 14(1):4. DOI: 10.1186/1480-9222-14-4
Source: PubMed

ABSTRACT Traditional analyses of calcium homeostasis have separately quantified either calcium accumulation or release mechanisms. To define the system as a whole, however, requires multiple experimental techniques to examine both accumulation and release. Here we describe a technique that couples the simultaneous quantification of radio-labeled calcium accumulation in endoplasmic reticulum (ER) microsomes with the release of inorganic phosphate (Pi) by the hydrolytic activity of sarco-endoplasmic reticulum calcium ATPase (SERCA) all in the convenience of a 96-well format.

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