HPTLC methods to assay active ingredients in pharmaceutical formulations: a review of the method development and validation steps.
ABSTRACT High-performance thin-layer chromatography (HPTLC) is still increasingly finding its way in pharmaceutical analysis in some parts of the world. With the advancements in the stationary phases and the introduction of densitometers as detection equipment, the technique achieves for given applications a precision and trueness comparable to high-performance liquid chromatography (HPLC). In this review, the literature is surveyed for developed and validated HPTLC methods to assay active ingredients in pharmaceutical formulations published in the period 2005-2011. Procedures and approaches for method development, validation and quantitative assays are compared with the standard ways of conducting them. Applications of HPTLC in some other areas are also briefly highlighted.
- SourceAvailable from: Palani Shanmugasundaram[show abstract] [hide abstract]
ABSTRACT: A validated HPTLC method for the estimation of piperine in herbal cough syrup is described. Separation was achieved on pre-coated silica gel plate 60F254 using Ethyl acetate: Hexane (60: 40 v/v) as mobile phase. Quantitation was carried out by the use of densitometer in absorbance mode at 330nm. The method gave good separation of piperine at Rf 0.37 from other compounds. The linearity for piperine was found to be in the concentration range of 10-50μg/ml. The percentage w/w content of piperine was found to ...Rasayan Journal of Chemistry 01/2008; 1(2):212-7.