Article

[Changes of glycogen synthase kinase-3β and its phospholation in spinal cord neurons in rats with incisional pain-remifentanil-induced hyperalgesia].

Department of Anesthesiology, Tianjin Medical University General Hospital, Tianjin, China.
Zhonghua yi xue za zhi 02/2012; 92(7):435-9.
Source: PubMed

ABSTRACT To investigate the change in glycogen synthase kinase-3β (GSK-3β) in spinal cord neurons in rats with incisional pain (IP)-remifentanil-induced hyperalgesia.
32 SD male rats (240 - 260 g) were randomly divided into 4 groups (n = 8 each): group R, group I, group R + I and group C. IP was established as Brennan's description. Paw withdrawal threshold (PWT) and paw withdrawal latency (PWL) were measured 24h before anesthesia and 2, 6, 24, 48 h after anesthesia. The rats were sacrificed after the last threshold measurement. The expressions of GSK-3β mRNA in rats' spinal cord neurons were determined by real-time PCR. The expressions of GSK-3β and pGSK-3β in rats' spinal cord neurons were determined by Western blot.
Remifentanil-induced hyperalgesia developed in group R, I and R + I. The expression of GSK-3β mRNA and the expression of GSK-3β in rats' spinal cord neurons were highest in group R + I. In addition, the ratio of pGSK-3β/GSK-3β was smallest in group R + I.
These data indicate that the increased GSK-3β activity in rats spinal cord neurons is involved in remifentanil-induced hyperalgesia.

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Keywords

32 SD male rats
 
4 groups
 
Brennan's description
 
glycogen synthase kinase-3β
 
group C. IP
 
group R
 
group R +
 
group R + I
 
GSK-3β mRNA
 
increased GSK-3β activity
 
IP)-remifentanil-induced hyperalgesia
 
last threshold measurement
 
paw withdrawal latency
 
Paw withdrawal threshold
 
rats spinal cord neurons
 
rats' spinal cord neurons
 
real-time PCR
 
Remifentanil-induced hyperalgesia
 
spinal cord neurons
 
Western blot