Article

DNA Origami Gatekeepers for Solid-State Nanopores

Walter Schottky Institute, Technische Universität München, Munich, Germany.
Angewandte Chemie International Edition (Impact Factor: 11.26). 05/2012; 51(20):4864-7. DOI: 10.1002/anie.201200688
Source: PubMed

ABSTRACT DNA has it covered: DNA origami gatekeeper nanoplates convert nanopores in solid-state membranes into versatile devices for label-free macromolecular sensing applications. The custom apertures in the nanoplates can be chemically addressed for sequence-specific detection of DNA.

Download full-text

Full-text

Available from: Ulrich Rant, Aug 26, 2015
0 Followers
 · 
125 Views
  • [Show abstract] [Hide abstract]
    ABSTRACT: Many processes in biology rely fundamentally on the relative position and orientation of interacting molecules. It is notoriously difficult to observe, let alone control, the position and orientation of molecules because of their small size and the constant thermal fluctuations that they experience. Molecular self-assembly with DNA origami enables building customshaped nanometer-scale objects with molecular weights in the megadalton regime. It provides a unique route for placing molecules and constraining their fluctuations in user-defined ways and thus opens up completely new avenues for scientific exploration.
    BioSpektrum 05/2012; 18(3). DOI:10.1007/s12268-012-0176-x
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Much more than ever, nucleic acids are recognized as key building blocks in many of life's processes, and the science of studying these molecular wonders at the single-molecule level is thriving. A new method of doing so has been introduced in the mid 1990's. This method is exceedingly simple: a nanoscale pore that spans across an impermeable thin membrane is placed between two chambers that contain an electrolyte, and voltage is applied across the membrane using two electrodes. These conditions lead to a steady stream of ion flow across the pore. Nucleic acid molecules in solution can be driven through the pore, and structural features of the biomolecules are observed as measurable changes in the trans-membrane ion current. In essence, a nanopore is a high-throughput ion microscope and a single-molecule force apparatus. Nanopores are taking center stage as a tool that promises to read a DNA sequence, and this promise has resulted in overwhelming academic, industrial, and national interest. Regardless of the fate of future nanopore applications, in the process of this 16-year-long exploration, many studies have validated the indispensability of nanopores in the toolkit of single-molecule biophysics. This review surveys past and current studies related to nucleic acid biophysics, and will hopefully provoke a discussion of immediate and future prospects for the field.
    Physics of Life Reviews 05/2012; 9(2):125-58. DOI:10.1016/j.plrev.2012.05.010 · 9.48 Impact Factor
  • Physics of Life Reviews 05/2012; 9(2):161-3; discussion 174-6. DOI:10.1016/j.plrev.2012.05.015 · 9.48 Impact Factor
Show more