Article

Photoactivation-based labeling and in vivo tracking of RNA molecules in the nucleus.

Cold Spring Harbor Protocols (Impact Factor: 4.63). 01/2006; 2006(6). DOI: 10.1101/pdb.prot4600
Source: PubMed

ABSTRACT This protocol describes a method for observing and measuring the movement of RNA molecules in the nucleus of living mammalian cells. Caged fluorescein-labeled DNA oligonucleotides are introduced into living mammalian cells, where they demonstrably hybridize to complementary RNA. After site-specific photoactivation at desired sites within the cell, the RNA movements away from those sites are followed and digitally recorded using a rapid acquisition microscopy system.

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