A serological investigation of bluetongue virus in cattle of south-east Iran

Department of Clinical Studies, School of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran. .
Veterinaria italiana (Impact Factor: 0.63). 01/2012; 48(1):41-4.
Source: PubMed


The aim of this study was to describe the seroprevalence rate of bluetongue virus (BTV) in cattle herds in south-east Iran. A total of 188 serum samples were collected from 20 cattle herds (10 animals in each herd) that were randomly selected between 2009 and 2010. A total of 12 samples were eliminated because of inadequacy. Antibodies to BTV in sera were detected using a commercial competitive enzyme-linked immunosorbent assay (c-ELISA). The seroprevalence rate in cattle was 2.13%. All sampled animals were female and age did not affect the prevalence of infection.

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    ABSTRACT: Objectives Bluetongue is a Culicoides-borne disease of domestic and wild ruminants. Bluetongue virus has rapid spread, irrespective of national borders, and is of major importance to the international trade of livestock. This virus did not isolate in Iran until 1974. The aim of the presentstudy was to review of epidemiological investigations of bluetongue diseasein Iran. Materials & methods Using Google scholar, full article or abstract of epidemiological investigations of bluetongue disease in different parts of Iran from 2003 to 2012 were collected. Data of location and date of study, evaluated species, isolation method(s), and prevalence of seropositive cases were descriptively analysed using the Microsoft Excel software (version 2010). Results& conclusion Fifteen studies were investigated bluetongue disease in the 11/31 provinces of Iran including Ardebil, Chaharmahal va Bakhtiari, East Azerbaijan, Fars, Ilam, Isfahan, Kerman, Khuzestan, Kurdistan, Qum, and West Azerbaijan from 2003 to 2012.Moreover, most of investigation were performed in the west frontier provinces, Kurdistan (6/15 studies), West Azerbaijan (4/15 studies), Khuzestan (4/15 studies), and Ilam (3/15 studies). The majority of researches were performed in sheep. Bluetongue virus was detected using competitive ELISA (12/15 studies), PCR (4/15 studies), and AGID (2/15 studies) methods. Distribution of publication of the studies did not uniform in the different years. Most of them were on 2008 (5/15 studies) and 2010(3/15studies).Prevalence of bluetongue disease in the seropositive ruminants (sheep, goats, cows, and camels) in the studies ranged from 0.84% to 100%. In conclusion, there is not adequate information about the distribution of bluetongue disease in most provinces of Iran or other domestic and wild ruminants of Iran. Pattern of rapid spread of bluetongue disease by Culicoides and its economic importance (decrease of herd production performance and increase of abortion rate) open a new window for Iranian researchers to investigate this disease.
    The 8th Convention of Iranian Veterinary Clinicians, Shiraz, Iran; 01/2013
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    ABSTRACT: Since 2000, several incursions of bluetongue virus (BTV) occurred in the Mediterranean Basin involving European and surrounding Countries. The Middle East represents one of the most important gateways for the access of BTV in Europe. Limited data on the BTV situation in this area are available. In this perspective, an epidemiological survey on the presence of BTV in Lebanon was conducted. Of the 181 serum samples tested, 97 (mean = 53.6%; 95% CI: 46.3-60.7) resulted positive when tested for the presence of BTV antibodies by c-ELISA, of these 42 (mean = 42%; 95% CI: 32.8-51.8) serum samples were from sheep and 55 (mean = 67.9%; 95% CI: 57.1-77.1) serum samples were from goats. Fourteen blood samples (14/110; mean = 12.7%; 95% CI: 7.8-20.3), 6 (6/66; mean = 9.1%; 95% CI: 4.4-18.5) from sheep and 8 (8/44; mean = 18.2%; 95% CI: 9.6-32.0) from goats, were positive by qRT-PCR. The results with serum-neutralization assay and typing performed by RT-PCR confirmed that six BTV serotypes are currently circulating in Lebanon, and these serotypes are as follows: 1, 4, 6, 8, 16 and 24. This study is the first report that confirms the presence and circulation of BTV in Lebanon.
    Transboundary and Emerging Diseases 07/2013; 60(5). DOI:10.1111/tbed.12126 · 2.94 Impact Factor
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    ABSTRACT: Aim: Bluetongue virus (BTV) is an economically important Orbivirus of the Reoviridae family, causes a haemorrhagic disease mainly in sheep and occasionally in cattle and some species of deer. The aim of this study was to describe the seroprevalence rate of bluetongue virus (BTV) in sheep in Kurdistan province, west of Iran. Also the history and epizootiology of bluetongue (BT) infection in Iran are reviewed. Study Design: method depends on Competitive Enzyme-Linked Immunosorbent Assay (c-ELISA). Place and Duration of Study: Departments of Veterinary, Agricultural and Natural Resources Research Center and Departments of Animal Virus Disease, Razi Vaccine and Serum Research Institute, in west of Iran during 2011-2012. Methodology: A total of 297 field sera collected from Healthy sheep with no symptoms in western Iran were screened for the present of group-specific BTV antibodies by competitive ELISA (c-ELISA). Results: The overall BTV antibodies prevalence was 42.42% in sheep (at 95% confidence level). Also a result was showed a significant increase in seroprevalence BT antibodies with classes of age. Conclusion: This investigation evaluates the present status of BT in western Iran. As well as the outbreak of BT in farm animals in the country was not recorded. A serological survey has indicated the presence of bluetongue virus (BTV) antibodies in sheep, goats, cattle and other farm animals in several states in Iran. However, clinical BT has not been observed in farm animals to date. BTV has not been isolated from Culicoides midges, although virus serotypes 3, 4, 9, 16, 20 and 22 were isolated in Iran.
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