Tumor ablation with nanosecond pulsed electric fields

Division of Hepatobiliary Pancreatic Surgery, First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China. .
Hepatobiliary & pancreatic diseases international: HBPD INT (Impact Factor: 1.17). 04/2012; 11(2):122-4. DOI: 10.1016/S1499-3872(12)60135-0
Source: PubMed
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    ABSTRACT: In the last decade, high-intensity pulsed electric fields with nanosecond durations (3-300 ns) have found breakthrough biomedical applications, e.g., in cancer treatment and gene therapy; however, the physical mechanisms underlying the interaction between nanosecond pulsed electric fields (nsPEFs) and cells, tissues, or organs are not yet fully elucidated. The precise knowledge of the electromagnetic dose received by the exposed sample at the macroscopic, and better still at the microscopic scale, is essential to complete our understanding of the phenomena involved and for adequate interpretation and reproducibility of the results. In this paper, we report a dosimetric and microdosimetric study of an in vitro exposure setup based on a transverse electromagnetic (TEM) cell that allows the exposure of cells in a Petri dish to nsPEFs. The rectangular and bipolar pulses delivered to the cells had a total duration of 1.2 ns and an amplitude of 2 kV. The electric field in situ was characterized experimentally with a nonmetallic probe and numerically using a finite-difference time-domain algorithm. Results of real-time monitoring of temperature were obtained at the subcellular level by using microfluorimetry, which is a method of imaging temperature by using a fluorescent molecular probe with thermosensitive properties.
    IEEE Transactions on Microwave Theory and Techniques 05/2013; 61(5):2015-2022. DOI:10.1109/TMTT.2013.2252917 · 2.24 Impact Factor
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    ABSTRACT: The cellular response to 100 ns pulsed electric fields (nsPEF) exposure includes the formation of transient nanopores in the plasma membrane and organelle membranes, an immediate increase in intracellular Ca(2+), an increase in reactive oxygen species (ROS), DNA fragmentation and caspase activation. 100 ns, 30 kV/cm nsPEF stimulates an increase in ROS proportional to the pulse number. This increase is inhibited by the anti-oxidant, Trolox, as well as the presence of Ca(2+) chelators in the intracellular and extracellular media. This suggests that the nsPEF-triggered Ca(2+) increase is required for ROS generation.
    Biochemical and Biophysical Research Communications 05/2013; 435(4). DOI:10.1016/j.bbrc.2013.05.014 · 2.30 Impact Factor
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    ABSTRACT: Nanosecond electric pulses (nsEP) are defined as very short high intensity electric pulses which present great potential for the destabilization of intracellular structures. Their theoretical descriptions first suggested specific effects on organelles that have been confirmed by various observations both in vitro and in vivo. However, due to their concomitant effects on the plasma membrane, nsEP can also affect cell functions. In this mini-review, nsEP effects on cells are described following three topics: effects at the plasma membrane level, intracellular effects, and the impact on cell survival. Eventually, a short description of the major results obtained in vivo will be presented. This study shows that the use of nsEP has evolved during the last decade to focus on low voltage for practical applications.
    Bioelectrochemistry 08/2014; 103. DOI:10.1016/j.bioelechem.2014.07.008 · 4.17 Impact Factor