Article

Reprogramming human endometrial fibroblast into induced pluripotent stem cells.

Department of Obstetrics and Gynecology, Taipei Veterans General Hospital, Taipei, Taiwan.
Taiwanese journal of obstetrics & gynecology 03/2012; 51(1):35-42. DOI:10.1016/j.tjog.2012.01.008 pp.35-42
Source: PubMed

ABSTRACT A recent breakthrough demonstrated that ectopic expression of four genes is sufficient to reprogram human fibroblasts into inducible pluripotent stem cells (iPSCs). However, it remains unknown whether human endometrial fibroblasts (EMFs) are capable of being reprogrammed into EMF-derived iPSCs (EMF-iPSCs).
EMFs were obtained from donors in their third and fourth decade of life and were reprogrammed into iPSCs using retroviral transduction with Oct-4, Sox2, Klf4, and c-Myc.
The EMF-iPSCs displayed the accelerated expression of endogenous Nanog and OCT-4 during reprogramming compared with EMFs. As a result, EMF-iPSC colonies that could be subcultured and propagated were established as early as 12 days after transduction. After 2 weeks of reprogramming, the human endometrial cells yielded significantly higher numbers of iPSC colonies and formed more 3D spheroid bodies than the EMFs. We have shown that human EMF-iPSCs are able to differentiate into neuronal-like cells, adipocytes, and osteocyte-like cells that express specific osteogenic genes.
Human EMFs can undergo reprogramming to establish pluripotent stem cell lines in female donors by the retroviral transduction of Oct-4, Sox2, Klf4, and c-Myc.

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Keywords

3D spheroid bodies
 
accelerated expression
 
ectopic expression
 
EMF-iPSC colonies
 
EMF-iPSCs
 
endogenous Nanog
 
express specific osteogenic genes
 
fourth decade
 
human EMF-iPSCs
 
Human EMFs
 
human endometrial cells
 
human endometrial fibroblasts
 
inducible pluripotent
 
iPSC colonies
 
neuronal-like cells
 
osteocyte-like cells
 
recent breakthrough
 
reprogram human fibroblasts
 
retroviral transduction
 
Sox2