Article

(1)H, (15)N and (13)C resonance assignments for the three LOTUS RNA binding domains of Tudor domain-containing protein TDRD7.

Department of Biochemistry and Molecular Biology, Mayo Clinic, Rochester, MN, 55905, USA.
Biomolecular NMR Assignments (impact factor: 0.72). 04/2012; DOI:10.1007/s12104-012-9382-1
Source: PubMed

ABSTRACT The LOTUS or OST-HTH domain is a recently discovered motif of about 80 amino acids and is found in several germline-specific proteins including the Tudor domain-containing proteins TDRD5 and TDRD7, which are important for germ cell development. The LOTUS domain is an RNA binding domain but its exact function is unknown. Here, we report the (1)H, (13)C and (15)N resonance assignments for the three LOTUS domains present in mouse TDRD7. These assignments will allow three-dimensional structure determination of the LOTUS domains and mapping of their interaction with RNA, steps toward deciphering the function of TDRD7.

0 0
 · 
0 Bookmarks
 · 
46 Views
Data provided are for informational purposes only. Although carefully collected, accuracy cannot be guaranteed. The impact factor represents a rough estimation of the journal's impact factor and does not reflect the actual current impact factor. Publisher conditions are provided by RoMEO. Differing provisions from the publisher's actual policy or licence agreement may be applicable.

Keywords

15)N resonance assignments
 
80 amino acids
 
exact function
 
germ cell development
 
LOTUS domains
 
mouse TDRD7
 
OST-HTH domain
 
TDRD7
 
three LOTUS domains present
 
Tudor domain-containing proteins TDRD5