Selective Chemical Imaging of Static Actin in Live Cells

Department of Chemical Biology, Max-Planck-Institute of Molecular Physiology, Dortmund, Germany.
Journal of the American Chemical Society (Impact Factor: 11.44). 04/2012; 134(20):8480-6. DOI: 10.1021/ja211708z
Source: PubMed

ABSTRACT We have characterized rationally designed and optimized analogues of the actin-stabilizing natural products jasplakinolide and chondramide C. Efficient actin staining was achieved in fixed permeabilized and non-permeabilized cells using different combinations of dye and linker length, thus highlighting the degree of molecular flexibility of the natural product scaffold. Investigations into synthetically accessible, non-toxic analogues have led to the characterization of a powerful cell-permeable probe to selectively image static, long-lived actin filaments against dynamic F-actin and monomeric G-actin populations in live cells, with negligible disruption of rapid actin dynamics.