Article

Direct quantification of γ-globulin in human blood serum by resonance light scattering techniques without separation of human serum albumin

College of Chemistry and Materials Science, Anhui Normal University, Wuhu 241000, PR China
Analytica Chimica Acta DOI:10.1016/S0003-2670(03)00872-9 pp.179-184

ABSTRACT We describe the preparation and characterization of functionalized nano-PbS. The functionalized nanoparticles are water-soluble. Reaction of mercaptoacetic acid functionalized nano-PbS with γ-globulin (γ-IgG) results in an enhanced resonance light scattering (RLS) at 385 nm. Based on this, a new direct quantitative method for γ-globulin in blood serum samples without separation of human serum albumin is established. Under optimal conditions, the enhanced RLS intensity is in proportion to the γ-IgG concentration in the range 10–500 ng ml−1. The 3σ limit of detection is 2.75 ng ml−1. The contents of γ-IgG in blood serum samples were determined with a recovery of 97–104% and R.S.D. of 1.5–2.1% (n=6). This method proved to be very sensitive, rapid, simple and tolerant of most interfering substances.

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Keywords

blood serum samples
 
contents
 
enhanced resonance light scattering
 
enhanced RLS intensity
 
human serum albumin
 
mercaptoacetic acid functionalized nano-PbS
 
new direct quantitative method
 
optimal conditions
 
rapid
 
substances