Article

Zinc oxide particles induce inflammatory responses in vascular endothelial cells via NF-κB signaling

Division of Environmental Health and Occupational Medicine, National Health Research Institutes, Zhunan, Miaoli County, Taiwan; Institute of Occupational Medicine and Industrial Hygiene, National Taiwan University, Taipei, Taiwan; Department of Environmental Science and Engineering, National Pingtung University of Science and Technology, Neipu, Pingtung, Taiwan; Center for Nanomedicine Research, National Health Research Institutes, Zhunan, Miaoli County, Taiwan
Journal of hazardous materials (Impact Factor: 4.33). 11/2010; DOI: 10.1016/j.jhazmat.2010.07.010

ABSTRACT This study investigated inflammatory effects of zinc oxide (ZnO) particles on vascular endothelial cells. The effects of 50 and 100-nm ZnO particles on human umbilical vein endothelial cells (HUVECs) were characterized by assaying cytotoxicity, cell proliferation, and glutathione levels. A marked drop in survival rate was observed when ZnO concentration was increased to 45 μg/ml. ZnO concentrations of ≤3 μg/ml resulted in increased cell proliferation, while those of ≤45 μg/ml caused dose-dependent increases in oxidized glutathione levels. Treatments with ZnO concentrations ≤45 μg/ml were performed to determine the expression of intercellular adhesion molecule-1 (ICAM-1) protein, an indicator of vascular endothelium inflammation, revealing that ZnO particles induced a dose-dependent increase in ICAM-1 expression and marked increases in NF-κB reporter activity. Overexpression of IκBα completely inhibited ZnO-induced ICAM-1 expression, suggesting NF-κB plays a pivotal role in regulation of ZnO-induced inflammation in HUVECs. Additionally, TNF-α, a typical inflammatory cytokine, induced ICAM-1 expression in an NF-κB-dependent manner, and ZnO synergistically enhanced TNF-α-induced ICAM-1 expression. Both 50 and 100-nm ZnO particles agglomerated to similar size distributions. This study reveals an important role for ZnO in modulating inflammatory responses of vascular endothelial cells via NF-κB signaling, which could have important implications for treatments of vascular disease.

0 Bookmarks
 · 
98 Views
  • [Show abstract] [Hide abstract]
    ABSTRACT: The aim of this study was to establish and identify the health effect markers of workers with potential exposure to nanoparticles (20–100 nm) during manufacturing and/or application of nanomaterials. For this cross-sectional study, we recruited 227 workers who handled nanomaterials and 137 workers for comparison who did not from 14 plants in Taiwan. A questionnaire was used to collect data on exposure status, demographics, and potential confounders. The health effect markers were measured in the medical laboratory. Control banding from the Nanotool Risk Level Matrix was used to categorize the exposure risk levels of the workers. The results showed that the antioxidant enzyme, superoxide dismutase (SOD) in risk level 1 (RL1) and risk level 2 (RL2) workers was significantly (p RL1 > RL2). Another antioxidant, glutathione peroxidase (GPX), was significantly lower only in RL1 workers than in the control workers. The cardiovascular markers, fibrinogen and ICAM (intercellular adhesion molecule), were significantly higher in RL2 workers than in controls and a significant dose–response with an increasing trend was found for these two cardiovascular markers. Another cardiovascular marker, interleukin-6, was significantly increased among RL1 workers, but not among RL2 workers. The accuracy rate for remembering 7-digits and reciting them backwards was significantly lower in RL2 workers (OR = 0.48) than in controls and a significantly reversed gradient was also found for the correct rate of backward memory (OR = 0.90 for RL1, OR = 0.48 for RL2, p Keywords: Antioxidant enzymes; Cardiovascular diseases; Control banding; Cross-sectional study; Environmental and health effects; Genotoxicity; Lung inflammation; Nanoparticle; Oxidative stress; Pulmonary function; Risk levels Document Type: Research Article DOI: http://dx.doi.org/10.1007/s11051-012-0878-5 Affiliations: 1: Division of Environmental Health & Occupational Medicine, National Health Research Institutes, 35 Keyan Road, Zhunan Town, Miaoli County, 35053, Taiwan, ROC, Email: shliou@nhri.org.tw 2: Division of Environmental Health & Occupational Medicine, National Health Research Institutes, 35 Keyan Road, Zhunan Town, Miaoli County, 35053, Taiwan, ROC 3: Department of Public Health, National Defense Medical Center, Taipei, Taiwan, ROC 4: Department of Chemistry, Fu Jen Catholic University, Taipei, Taiwan, ROC 5: Institute of Occupational Safety and Health, Council of Labor Affairs, Taipei, Taiwan, ROC 6: Institute of Environmental Health, College of Public Health, China Medical University and Hospital, Taichung, Taiwan, ROC Publication date: August 1, 2012 $(document).ready(function() { var shortdescription = $(".originaldescription").text().replace(/\\&/g, '&').replace(/\\, '<').replace(/\\>/g, '>').replace(/\\t/g, ' ').replace(/\\n/g, ''); if (shortdescription.length > 350){ shortdescription = "" + shortdescription.substring(0,250) + "... more"; } $(".descriptionitem").prepend(shortdescription); $(".shortdescription a").click(function() { $(".shortdescription").hide(); $(".originaldescription").slideDown(); return false; }); }); Related content In this: publication By this: publisher In this Subject: Nuclear Physics By this author: Liou, Saou-Hsing ; Tsou, Tsui-Chun ; Wang, Shu-Li ; Li, Lih-Ann ; Chiang, Hung-Che ; Li, Wan-Fen ; Lin, Pin-Pin ; Lai, Ching-Huang ; Lee, Hui-Ling ; Lin, Ming-Hsiu ; Hsu, Jin-Huei ; Chen, Chiou-Rong ; Shih, Tung-Sheng ; Liao, Hui-Yi ; Chung, Yu-Teh GA_googleFillSlot("Horizontal_banner_bottom");
    Journal of Nanoparticle Research 01/2012; 14(8). · 2.18 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Objectives: The purpose of this study is to examine whether Hominis Placental pharmacopuncture solution (HPPS) combined with zinc-oxide nanoparticles (ZnO NP) activates RAW 264.7 cells. Methods: We soaked ZnO nanoparticles in the Hominis Placenta pharmacopuncture solution, thereby making a combined form (ZnO NP HPPS). The effect of ZnO NP HPPS on the intracellular reactive oxygen species (ROS) production was measured by 2', 7'-dichlorofluorescin diacetate (DCFH-DA) assay. The effect of ZnO NP HPPS on NF- was measured by using a luciferase assay. The effect of ZnO NP HPPS on the cytokine expression was assessed by semi-quantitative reverse transcriptase polymerase chain reaction (RT-PCR). The cellular uptake of ZnO NP HPPS was measured by using a flow cytometric analysis, and cellular structural alterations were analyzed by using transmission electron microscopy (TEM). Results: Neither the HPPS nor the ZnO NPs induced intracellular ROS production in RAW 264.7 cells. Neither of the materials activated NF- or it's dependent genes, such as TNF-, IL-1, and MCP-1. However, ZnO NP HPPS, the combined form of ZnO NPs and HPPS, did induce the intracellular ROS production, as well as prominently activating NF- and it's dependent genes. Also, compared to ZnO NPs, it effectively increa-sed the uptake by RAW 264.7 cells. In addition, cellular structural alterations were observed in groups treated with ZnO NP HPPS. Conclusions: Neither ZnO NP nor HPPS activated RAW 264.7 cells, which is likely due to a low cellular uptake. The ZnO NP HPPS, however, significantly activated NF- and up-regulated its dependent genes such as TNF-, IL-1, and MCP-1. ZnO NP HPPS was also more easily taken into the RAW 264.7 cells than either ZnO NP or HPPS.
    Journal of Pharmacopuncture. 01/2012; 15(3).
  • [Show abstract] [Hide abstract]
    ABSTRACT: Zinc oxide nanoparticles are highly sensitive towards phosphate ions even at pH 7. Buffer solutions and cell culture media containing phosphate ions are able to destroy ZnO nanoparticles within a time span from less than one hour to one day. The driving force of the reaction is the formation of zinc phosphate of very low solubility. The morphology of the zinc oxide particles has only a minor influence on the kinetics of this reaction. Surface properties related to different production methods and the presence and absence of labelling with a perylene fluorescent dye are more important. Particles prepared under acidic conditions are more resistant than those obtained in basic or neutral reaction medium. Surprisingly, the presence of a SiO2 coating does not impede the degradation of the ZnO core. In contrast to phosphate ions, β-glycerophosphate does not damage the ZnO nanoparticles. These findings should be taken into account when assessing the biological effects or the toxicology of zinc oxide nanoparticles.
    Beilstein Journal of Nanotechnology 01/2014; 5:2007-15. · 2.37 Impact Factor