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Reclassification of a pneumocandin-producing anamorph, Glarea lozoyensis gen. et sp. nov., previously identified as Zalerion arboricola

Natural Products Drug Discovery, Merck Research Laboratories, Rahway, New Jersey, 07065-0900, U.S.A.
Mycological Research (Impact Factor: 2.81). 02/1999; DOI: 10.1017/S095375629800687X

ABSTRACT The importance of pneumocandin BO as the fermentation-derived starting material for the antifungal drug candidate, MK-991, along with the identification of our production strain as Z. arboricola (ATCC 20868) as CBS prompted a search for other strains of Z. arboricola or Zalerion species with improved titres or that might produce natural pneumocandin analogues. Analysis of morphology, secondary metabolites profiles, and DNA fingerprinting demonstrated that ATCC 20868 was not congeneric with Z. arboricola. Ribosomal DNA sequences were compared among Zalerion species and pneumocandin-producing fungi and with rDNA sequences in GenBank. No good matches with sequences in GenBank were obtained for Z. arboricola or Z. maritimum, but for Z. varium, P. carpinea and ATCC 20868, relevant similarities were observed with ITS1 sequences from fungi of Leotiales. ATCC 20868 was phylogenetically more akin to P. carpinea, another pneumocandin producer, than initially suspected. The closest relative of ATCC 20868 seemed to be Hymenoscyphus monotropae. We conclude that the genus Zalerion is artificial; its species bear no phylogenetic relation among themselves. ATCC 20868 and Z. varium were related to fungi of the Leotiales. We propose a new anamorph genus and species, Glarea lozoyensis, to accommodate ATCC 20868.

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Available from: Gerald F Bills, Feb 27, 2015
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    • "Nine strains forming sterile, dense, brown aerial mycelium shared 97–99% similarity of their ITS rDNA sequences. In a partial analysis (data not shown), these strains fell into two moderately supported clades that were sister to sequence AF169307 from the ex-type strain of Zalerion arboricola (Bills et al. 1999). After 1 year of cultivation at the lowered temperature, the strains began to produce conidia that were similar in shape and size to those of Z. arboricola, which points to higher variability in the ITS rDNA of Z. arboricola rather than to a new species. "
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