Effect of sucrose concentrations on somatic embryogenesis in carnation (Dianthus caryophyllus L.)
ABSTRACT The effect of sucrose concentration on callus induction followed by differentiation of embryogenic callus derived from petal explants of four carnation cultivars (Nelson, Sagres, Spirit and Impulse) was investigated. Embryogenic calli were produced on Murashige and Skoog [Murashige, T., Skoog, F.A., 1962. Revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant 154, 73–479] basal medium (MS) culture medium containing six concentrations of sucrose (3, 6, 9, 12, 15 and 18%, w/v) all supplemented with 9 μM 2,4-dichlorophenoxyacetic acid (2,4-D) and 0.8 μM 6-benzyladenine (BA). Maximum frequency of embryogenic callus was obtained from the media containing 9 and 12% sucrose. Somatic embryos were induced on a hormone-free MS media containing the seven concentrations of sucrose. Development of somatic embryos was enhanced by increasing sucrose concentration from 1.5 to 12%, while it was reduced in higher concentrations of 15 and 18%. However, normal embryos were not developed in the media containing 1.5 and 3% sucrose. Ninety-five percent of somatic embryos were regenerated to form the entire plantlets when they transferred onto the half-strength hormone-free MS culture medium containing 3% sucrose. Plantlets were also continued to grow normally under greenhouse condition.
- SourceAvailable from: Subhash J BhoreMiddle East Journal of Scientific Research 01/2010; 6:418-428.
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ABSTRACT: Somatic embryogenesis was obtained from callus of wild arum (Arum palaestinum). Callus was induced from sterilized corm bud sprouts cultured on basal medium containing 4.4 μM 6-benzyladenine and 5.4 μM 1-naphthaleneacetic acid. Callus was maintained under dark conditions using basal medium with 4.4 or 8.8 μM 6-benzyladenine and 5.4 or 10.8 μM 1-naphthaleneacetic acid. The highest callus weight and most desirable callus phenotype were achieved using basal medium containing 8.8 μM 6-benzyladenine and 5.4 μM 1-naphthaleneacetic acid. Friable calli were cultured in the dark on basal medium containing 4.5 μM 2, 4-dichlorophenoxyacetic acid, 0.46 μM 6-furfurylaminopurine, 5.4 μM 1-naphthaleneacetic acid, and 1.7 mM proline to induce embryogenesis before transfer to regeneration medium. Embryos that developed on regeneration medium were transferred to medium minus plant growth regulators for germination. Ninety percent of the germinating embryos developed into rooted plantlets. Rooted plants were grown in the greenhouse and acclimatized successfully with a 95 % survival rate. This is the first report of successful somatic embryogenesis and plant regeneration in A. palaestinum.In Vitro Cellular & Developmental Biology - Plant 06/2012; 48(3). · 1.16 Impact Factor
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ABSTRACT: Competence of two apple rootstocks M. 9 and M. 26 for in vitro shoot proliferation was appraised using a miscellany of carbon sources i.e., sorbitol, sucrose, glucose and mannitol which were employed @ 0, 5, 15, 25, 35 and 45 g l -1 . The most auspicious outcome was achieved by sorbitol @ 35 g l -1 (T 9) being the optimal carbon source for both the genotypes. M. 26 had a positive interaction with sorbitol at this concentration to produce the best caulogenic response in terms of a paramount shoot length (3.01 cm) and an overriding fresh weight increment (402 mg) whereas M. 9 at the same concentration gave an eminent shoot number (9.8). Sucrose and glucose also had a positive carryover effect on apple shoots to some extent but proved to be inferior to sorbitol. Results yielded by mannitol were highly indigent in comparison to other carbon sources. Rootstocks exhibited an inconsistency regarding their aptitude for shoot proliferation. M. 26 was recognized as a better rootstock with an acquisition of 1.05 cm shoot length and 154.6 mg fresh weight while M. 9 stood better with maximum shoot number of 2.3.