Article

Synthesis, biodegradability and cytotoxicity of water-soluble isobutylchitosan

State Key Laboratory of Trauma, Burn and Combined Injury, The 2nd Department of Research Institute of Surgery, The Military Medical University, Chongqing 400042, China
Carbohydrate Polymers DOI:10.1016/j.carbpol.2006.04.022 pp.40-45

ABSTRACT The objective of the present study was to improve the solubility of chitosan at neutral aqueous solution without altering its bioactivity. Through an alkylation reaction under mild conditions, isobutyl was introduced to chitosan to form isobutylchitosan, which showed a good solubility in neutral aqueous media resulting from the reduced crystallinity of the product. The structure of isobutylchitosan was characterized by IR, 1H NMR spectroscopy, and X-ray diffraction. The degradation of the product was evaluated in vivo using a mouse model, and the cytotoxicity of the product in L929 mouse fibroblasts was monitored using the MTT assay and the release of the cytosolic enzyme lactate dehydrogenase (LDH). It was found that the product exhibited the properties of improved biodegradability, increased MTT values and decreased LDH release. The results suggested that isobutylchitosan might be one of promising and safe biomaterial with potential applications.

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Keywords

1H NMR spectroscopy
 
altering
 
biodegradability
 
cytosolic enzyme lactate dehydrogenase
 
form isobutylchitosan
 
LDH release
 
mild conditions
 
mouse model
 
neutral aqueous media
 
neutral aqueous solution
 
potential applications
 
product exhibited
 
promising
 
reduced crystallinity
 
safe biomaterial
 
vivo
 
X-ray diffraction