Rapid estimation of sulfide in rumen and blood with a sulfide-specific ion electrode

Chemistry and Biology Research Institute Research Branch, Agriculture Canada, Ottawa, Ontario K1A 0C6, Canada; Animal Research Institute, Research Branch, Agriculture Canada, Ottawa, Ontario K1A 0C6, Canada
Microchemical Journal (Impact Factor: 3.58). 09/1980; DOI: 10.1016/0026-265X(80)90280-5

ABSTRACT Free sulfide in rumen preserved with a sulfide antioxidation reducing buffer (SAOB) is determined directly and rapidly with a sulfide ion electrode using a standard addition technique. Acid-labile sulfide in blood preserved in alkaline cadmium acetate is determined by electrode measurement after acid liberation in a Johnson-Nishita apparatus and absorption in 50% SAOB. The sulfide antioxidant reducing buffer SAOB is not recommended for preservation of blood samples because of its desulfuration effect on S-proteins and S-amino acids present in blood.

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