Article

A Comparison of Peronospora parasitica (Downy Mildew) Isolates from Arabidopsis thaliana and Brassica oleracea Using Amplified Fragment Length Polymorphism and Internal Transcribed Spacer 1 Sequence Analyses

Horticulture Research International, Wellesbourne, Warwickshire, CV35 9EF, United Kingdom
Fungal Genetics and Biology DOI:10.1006/fgbi.2000.1216 pp.95-103

ABSTRACT Amplified fragment length polymorphism (AFLP) fingerprints and internal transcribed spacer 1 (ITS1) sequences from 27 Peronospora parasitica isolates (collected from Arabidopsis thaliana or Brassica oleracea), 5 Albugo candida isolates (from the same hosts and from Capsella bursa-pastoris), and 1 Bremia lactucae isolate (from Lactuca sativa) were compared. The AFLP analysis divided the isolates into five groups that correlated with taxonomic species and, in most cases, with host origin. The only exception was a group consisting of A. candida isolates from both B. oleracea and C. bursa-pastoris. ITS1 sequence analysis divided the isolates into the same five groups, demonstrated the divergence between P. parasitica isolates from A. thaliana and B. oleracea, and, using previously published ITS1 sequences, clearly showed the relationship between A. candida isolates from different hosts.

0 0
 · 
0 Bookmarks
 · 
25 Views
Data provided are for informational purposes only. Although carefully collected, accuracy cannot be guaranteed. The impact factor represents a rough estimation of the journal's impact factor and does not reflect the actual current impact factor. Publisher conditions are provided by RoMEO. Differing provisions from the publisher's actual policy or licence agreement may be applicable.

Keywords

1 Bremia lactucae
 
27 Peronospora parasitica
 
A. candida
 
A. thaliana
 
AFLP analysis
 
Amplified fragment length polymorphism
 
Arabidopsis thaliana
 
Capsella bursa-pastoris
 
cases
 
different hosts
 
divergence
 
internal transcribed spacer 1
 
ITS1
 
ITS1 sequence analysis
 
ITS1 sequences
 
taxonomic species