Article

Rapid and reliable identification of Streptococcus anginosus group isolates to the species level by real-time PCR and melting curve analysis

Department of Internal Medicine, Canisius Wilhelmina Hospital, Nijmegen, The Netherlands; Department of Medical Microbiology & Infectious Diseases, Canisius Wilhelmina Hospital, Nijmegen, The Netherlands; Department of Medical Microbiology, University Hospital Maastricht, The Netherlands
Journal of Microbiological Methods DOI:10.1016/j.mimet.2008.06.022 pp.372-374

ABSTRACT A real-time PCR assay is described for rapid and accurate identification of the Streptococcus anginosus group members. Based on melting curve analysis, the S. anginosus species isolates could be further subdivided into two subgroups, each associated with different clinically relevant ribotypes of S. anginosus.

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Keywords

accurate identification
 
curve analysis
 
different clinically relevant ribotypes
 
real-time PCR assay