Expression analysis of RING zinc finger genes from Triticum aestivum and identification of TaRZF70 that contains four RING-H2 domains and differentially responds to water deficit between leaf and root
ABSTRACT RING zinc finger proteins are known for their role predominantly in targeted protein degradation and participate in gene regulation through interaction with other regulatory proteins. In this study seven RING zinc finger genes from Triticum aestivum (bread wheat) were analysed for expression profiles in various organs (leaf, root, stem, spike, endosperm and embryo) and during leaf development and aging as well as in their responses to water deficit. Expression levels of six of these seven genes varied markedly among the six organs examined. All seven genes changed their expression levels in the leaf from the growing to senescing stage. Four genes were responsive to water deficit. A RING-H2 zinc finger gene, TaRZF70 showed differential response to water deprivation, namely up-regulation in the leaf and down-regulation in the root. This differential response was also observed in abscisic acid (ABA)-treated plants. Sequence analysis revealed that TaRZF70 contained four RING-H2 domains, the largest number of RING-H2 domains in any RING-H2 zinc finger proteins reported to date. These results indicate that these RING zinc finger genes are involved in diverse physiological processes in wheat, including response to drought.
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ABSTRACT: Zinc finger proteins are encoded by the genes chiefly involved in stress resistance hormone signal transduction of plants. In this study, a cDNA encoding a zinc finger transcription factor was isolated by the yeast one-hybrid system from Gossypium arboreum using the MYB-box element of the universal stress gene (GUSP1) promoter as bait. The corresponding protein (named GaZnF) can bind specifically to a 13 bp MYB-box region. The GaZnF cDNA is 1093 bp in length, including a 510 bp open reading frame. The predicted GaZnF protein contains ANI-A20 motifs and shares a high sequence similarity with zinc finger proteins from other plants. Spatial expression pattern of GaZnF was studied under drought, heavy metals and salt stresses through real-time PCR. The gene showed enhanced expression under each stress treatment with maximum transcript abundance in root tissues. The results support the hypothesis that G. arboreum zinc finger proteins are involved in plant response to drought, salt and heavy metal stresses.Biologia Plantarum 56(4). · 1.69 Impact Factor
Article: Drought Tolerance in Wheat.[Show abstract] [Hide abstract]
ABSTRACT: Drought is one of the most important phenomena which limit crops' production and yield. Crops demonstrate various morphological, physiological, biochemical, and molecular responses to tackle drought stress. Plants' vegetative and reproductive stages are intensively influenced by drought stress. Drought tolerance is a complicated trait which is controlled by polygenes and their expressions are influenced by various environmental elements. This means that breeding for this trait is so difficult and new molecular methods such as molecular markers, quantitative trait loci (QTL) mapping strategies, and expression patterns of genes should be applied to produce drought tolerant genotypes. In wheat, there are several genes which are responsible for drought stress tolerance and produce different types of enzymes and proteins for instance, late embryogenesis abundant (lea), responsive to abscisic acid (Rab), rubisco, helicase, proline, glutathione-S-transferase (GST), and carbohydrates during drought stress. This review paper has concentrated on the study of water limitation and its effects on morphological, physiological, biochemical, and molecular responses of wheat with the possible losses caused by drought stress.The Scientific World Journal 01/2013; 2013:610721. · 1.73 Impact Factor
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ABSTRACT: Really interesting new gene (RING)-H2 proteins are widely represented in plants and play important roles in the regulation of many developmental processes as well as in plant–environment interactions. In the present report, a full-length cDNA encoding a putative RING-H2 protein with high sequence homology to Arabidopsis thaliana RING-H2 gene XERICO, designated as ZmXERICO, was isolated from maize. The full-length cDNA of ZmXERICO contained a 435 bp open reading frame encoding a precursor protein of 145 amino acid residues. Comparative and bioinformatics analyses revealed that ZmXERICO showed a high degree of homology with RING-H2 proteins and contained the transmembrane domain (TM) and RING-H2 motif. In our study, ZmXERICO was analyzed for expression profiles in various organs (root, stem, leaf, stamen, pistil and seed) and responding to exogenous hormone treatment and abiotic stresses using real-time PCR. The results showed that the expression of ZmXERICO was different regulated after treated by gibberellins acid-3 (GA3) and GA biosynthetic inhibitor paclobutrazol between in light and darkness. Moreover, the expression of ZmXERICO under different abiotic stresses was analyzed at different time points. The ZmXERICO was up-regulated in salt, drought, abscisic acid and cold stress. These expression results suggested that ZmXERICO played an important role in plant hormones signal pathways and light signal pathways, and that ZmXERICO might be involved in plant defense system against environmental stresses in maize.Acta Physiologiae Plantarum 34(4). · 1.31 Impact Factor