Article

Kinetic enzymatic method for automated determination of glucose in blood and serum.

ABSTRACT Studies are reported on the reaction kinetics of the glucose assay according to Trinder which involves the specific oxidation of glucose by glucose oxidase and the determination of the hydrogen peroxide released by means of phenol and 4-aminophenazone in the presence of peroxidase. The results have been used to develop a general kinetic fixed-time method for the analysis of glucose in whole blood and serum. The single reagent method has been adapted to the ENI GEMSAEC centrifugal analyzer and to the Abbott ABA-100 analyzer. The procedures exhibited excellent precision and the results correlated well with those obtained by the hexokinase method, Linearity was achieved from 3 to 64 mmol/1 glucose for the GEMSAEC method, and from 3 to 33 mmol/1 glucose for the ABA-100 method. Reagent or sample blank corrections were not necessary. There were no interferences from various drugs, hemoglobin, bilirubin, or lipemia.

1 Bookmark
 · 
347 Views
  • [Show abstract] [Hide abstract]
    ABSTRACT: The present study was aimed to investigate the metabolic effects of acute and chronic oral administration of maltitol (4-O-i-D-glucopyranosyl-D-glucitol), a hypocaloric sweetener obtained from maltose by catalytic hydrogenation. Fifty grams of maltitol induced a increase lower glycemic and insulinemic than the same dose of glucose or sucrose. No variations of plasma glucose and serum insulin levels were observed after 180 min. A slight increase of plasma maltitol was observed 45 min after maltitol. The chronic administration of maltitol (10 g 3 X daily for 5 days) induced no variations of glycemia or insulinemia when compared with the same dose of sucrose. Plasma sorbitol levels were slightly higher after maltitol than after sucrose. Low amounts of maltitol were detected in the urine and feces.
    Klinische Wochenschrift 04/1986; 64(6):265-9.
  • [Show abstract] [Hide abstract]
    ABSTRACT: The aim of this study is to assess in human vein endothelial cells cultures the variations related to aging in: number of cells, glucose, triglycerides, thiobarbituric acid reactive substances (TBARS), calcium, phosphorus, cholesterol, total protein and urea and to analyze the relation of these parameters between themselves. Eight parallel flasks were subjected to the natural aging process, one of them without cells, analyzed at eight chronological stages. The determinations of biochemical parameters were performed in culture medium when it was changed. The number of cells, glucose, triglycerides and TBARS show significant variations during the aging process (P < 0.001). The glucose uptake per cell is maintained relatively constant in time (0.9 ng/cell day). A high linear correlation between glucose and the number of cells (r=0.935), triglycerides and the number of cells (r=0.505), triglycerides and decrease in number of cells (r=0.805) is observed. The measurement of glucose uptake seems to be an excellent method for the assessment of energetic cellular metabolism and, in certain conditions, can be used to estimate the number of cells. In the latest stages of aged cells a slight increase in glucose uptake has been observed. There is an increase of triglycerides and thiobarbituric acid reactive substances release during aging process.
    Mechanisms of Ageing and Development 06/1998; 103(1):13-26. · 3.26 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: We describe a rapid, kinetic, fixed-time method for determining serum total cholesterol by use of cholesterol esterase, cholesterol oxidase, and the indicator reaction with peroxidase, 4-aminophenazone, and phenol. On addition of the competitive inhibitor 3,4-dichlorophenol the Michaelis constant of cholesterol oxidase is apparently increased, which extends the linear relation between absorbance change and cholesterol concentration to 20.7 to 25.9 mmol/L, depending on the analyzer being used. For calibration, a single standard is used. Total analysis time is in the range of 80 to 210 s. Incubation temperature is 25 degrees C or 37 degrees C. The single-reagent procedure has been adapted to three different centrifugal analyzers and to the Eppendorf ACP 5040 analyzer. It yields precise and accurate results and is insensitive to potential interferences.
    Clinical Chemistry 11/1983; 29(10):1798-802. · 7.15 Impact Factor

Full-text

Download
3 Downloads
Available from