A Butyl Methacrylate Monolithic Column Prepared In-Situ on a Microfluidic Chip and its Applications

National Key Disciplines Lab of Novel Micro-Nano Devices and System Technology, and International R&D Center of Micro-Nano Systems and New Materials Technology, and Microsystem Research Center, and College of Chemistry and Chemical Engineering, Chongqing University, Chongqing, 400044, China
Sensors (Impact Factor: 2.25). 05/2009; 9(5):3437-46. DOI: 10.3390/s90503437
Source: PubMed


A butyl methacrylate (BMA) monolithic column was polymerized in-situ with UV irradiation in an ultraviolet transparent PDMS micro-channel on a homemade micro-fluidic chip. Under the optimized conditions and using a typical polymerization mixture consisting of 75% porogenic solvents and 25% monomers, the BMA monolithic column was obtained as expected. The BET surface area ratio of the BMA monolithic column was 366 m(2)·g(-1). The corresponding SEM images showed that the monolithic column material polymerized in a glass channel was composed of uniform pores and spherical particles with diameters ranging from 3 to 5 μm. The promethazine-luminal-potassium ferricyanide chemiluminescence system was selected for testing the capability of the column. A flow injection analytical technique-chemiluminescence (FIA-CL) system on the microfluidic chip with a BMA monolithic column pretreatment unit was established to determine promethazine. Trace promethazine was enriched by the BMA monolithic column, with more than a 10-fold average enrichment ratio. The proposed method has a linear response concentration range of 1.0×10(-8) - 1.0×10(-6)g·mL(-1) and the detection limit was 1.6×10(-9)g·mL(-1).

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