Article

A map of the distal region of the long arm of human chromosome 21 constructed by radiation hybrid mapping and pulsed-field gel electrophoresis

Department of Physiology, University of California, San Francisco, California 94143 USA; Department of Psychiatry, University of California, San Francisco, California 94143 USA; Department of Biochemistry and Biophysics, University of California, San Francisco, California 94143 USA; Department of Microbiology and Immunology, University of California, San Francisco, California 94143 USA; Genetics Division, Children's Hospital, Harvard University School of Medicine, Boston, Massachussetts 02115 USA
Genomics (Impact Factor: 3.01). 02/1991; DOI: 10.1016/0888-7543(91)90216-2

ABSTRACT We have used radiation hybrid (RH) mapping and pulsedfield gel electrophoresis (PFGE) to determine the order and positions of 28 DNA markers from the distal region of the long arm of human chromosome 21. The maps generated by these two methods are in good agreement. This study, combined with that of D. R. Cox et al. (1990, Science 250:245–250), results in an RH map that covers the long arm of chromosome 21 (21q). We have used a subtelomeric probe to show that our map includes the telomere and have identified single-copy genes and markers within 200 kbp of the telomere. Comparison of the physical and RH maps with genetic linkage maps shows “hot spots” of meiotic recombination in the distal region, one of which is close to the telomere, in agreement with previous cytogenetic observations of increased recombination frequency near telomeres.

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