A map of the distal region of the long arm of human chromosome 21 constructed by radiation hybrid mapping and pulsed-field gel electrophoresis
ABSTRACT We have used radiation hybrid (RH) mapping and pulsedfield gel electrophoresis (PFGE) to determine the order and positions of 28 DNA markers from the distal region of the long arm of human chromosome 21. The maps generated by these two methods are in good agreement. This study, combined with that of D. R. Cox et al. (1990, Science 250:245–250), results in an RH map that covers the long arm of chromosome 21 (21q). We have used a subtelomeric probe to show that our map includes the telomere and have identified single-copy genes and markers within 200 kbp of the telomere. Comparison of the physical and RH maps with genetic linkage maps shows “hot spots” of meiotic recombination in the distal region, one of which is close to the telomere, in agreement with previous cytogenetic observations of increased recombination frequency near telomeres.
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ABSTRACT: Neuropathological abnormalities in Down syndrome are reviewed and reported. Among these are subtle but characteristic alterations in brain growth and development most apparent after 1 year of age. Neurons are also altered in Down syndrome. There are reductions in total dendritic length and number of spines begining at around 4 months of age. In addition, the number of the S-100 protein positive cells is increased in the perinatal period correlating perhaps with increased expression in Down syndrome because of the presence of three chromosome 21s. Down syndrome is a complete disorder which can only be analyzed by utilizing a wide variety of techniques.Neuropathology 01/1993; 13(4):295-299. · 1.91 Impact Factor
- Immunogenetics 01/1991; 34(5):341-345. · 2.89 Impact Factor
- Studies in Surface Science and Catalysis - STUD SURF SCI CATAL. 01/1996; 99:357-409.