Article

Formation of chromosome aberrations: insights from FISH

Department Toxicogenetics, Leiden University Medical Center, Wassenaarseweg 72, 2333 AL Leiden, The Netherlands
Mutation Research/Reviews in Mutation Research DOI:10.1016/j.mrrev.2003.09.005 pp.299-304

ABSTRACT Most of the mutagenic and carcinogenic agents induce chromosome aberrations in vivo and in vitro. Conventional solid staining (such as Giemsa) has been employed to evaluate the frequencies and types of spontaneous and induced chromosomal aberrations. Recently, molecular cytogenetic techniques such as fluorescence in situ hybridization (FISH) using chromosome specific or chromosome region-specific DNA libraries have become available, which have increased the resolution of the detection of aberrations. This has lead to a better understanding on the mechanisms of formation of chromosome aberrations, especially following treatment with ionizing radiation. The present paper reviews briefly the results obtained using FISH technique both from basic and applied studies.

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Keywords

carcinogenic agents induce chromosome aberrations
 
chromosome region-specific DNA libraries
 
Conventional solid staining
 
detection
 
FISH technique
 
induced chromosomal aberrations
 
ionizing radiation
 
present paper reviews
 
situ hybridization
 
vitro
 

A T Natarajan