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Chapter Thirteen - Magnetic Tweezers for the Study of DNA Tracking Motors

Laboratoire de Physique Statistique, Ecole Normale Superieure, Université Paris Diderot, Paris, France; Département de Biologie , Ecole Normale Superieure, Paris, France; Department of Chemistry, The Pennsylvania State University, University Park, Pennsylvania, USA; CNRS, Centre de Génétique Moléculaire, Gif-sur-Yvette, France; Université Paris-Sud, Orsay, Paris, France; Materials Department and BMSE Program, University of California, Santa Barbara, California, USA; Institut Universitaire de France, Paris, France; Department of Chemistry and Biochemistry, UCLA, Los Angeles, California, USA
Methods in Enzymology DOI:10.1016/S0076-6879(10)75013-8 pp.297-320

ABSTRACT Single-molecule manipulation methods have opened a new vista on the study of molecular motors. Here we describe the use of magnetic traps for the investigation of the mechanism of DNA based motors, in particular helicases and translocases.

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Keywords

magnetic traps
 
molecular motors
 
new vista
 
Single-molecule manipulation methods