Polymerase chain reaction assay for verifying the labeling of meat and commercial meat products from game birds targeting specific sequences from the mitochondrial D-loop region

Departamento de Nutrición, Bromatología y Tecnología de los Alimentos Facultad de Veterinaria, Universidad Complutense, 28040 Madrid, Spain.
Poultry Science (Impact Factor: 1.67). 05/2010; 89(5):1021-1032. DOI: 10.3382/ps.2009-00217

ABSTRACT A PCR assay was developed for the identification of meats and commercial meat products from quail (Coturnix coturnix), pheasant (Phasianus colchicus), partridge (Alectoris spp.), guinea fowl (Numida meleagris), pigeon (Columba spp.), Eurasian woodcock (Scolopax rusticola), and song thrush (Turdus philomelos) based on oligonucleotide primers targeting specific sequences from the mitochondrial D-loop region. The primers designed generated specific fragments of 96, 100, 104, 106, 147, 127, and 154 bp in length for quail, pheasant, partridge, guinea fowl, pigeon, Eurasian woodcock, and song thrush tissues, respectively. The specificity of each primer pair was tested against DNA from various game and domestic species. In this work, satisfactory amplification was accomplished in the analysis of experimentally pasteurized (72 degrees C for 30 min) and sterilized (121 degrees C for 20 min) meats, as well as in commercial meat products from the target species. The technique was also applied to raw and sterilized muscular binary mixtures, with a detection limit of 0.1% (wt/wt) for each of the targeted species. The proposed PCR assay represents a rapid and straightforward method for the detection of possible mislabeling in game bird meat products.

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    • "TaqMan real-time PCR systems based on nucleotide sequence variation in D-loop and 12S rRNA mitochondrial genes were developed for the detection and quantification of DNA from chicken, turkey, duck, and goose material in highly processed industrial feed samples (Pegels et al., 2012). Rojas et al. (2010), targeted the mitochondrial 12S rRNA gene for the identification of meats and commercial meat products from game birds, including quail, pheasant, partridge, guinea fowl, pigeon, Eurasian woodcock and song thrush. "
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