Article

Development of a shear force scanning near-field fluorescence microscope for biological applications

LMEN, (EA), UFR des Sciences, Université de Reims Champagne-Ardenne, 21 rue Clément Ader, 51685 Reims Cédex 2, France; Unité MéDIAN, CNRS-UMR 6142, UFR de Pharmacie, IFR 53, Université de Reims Champagne-Ardenne, 51 rue Cognacq Jay, 51096 Reims Cédex, France
Ultramicroscopy DOI:10.1016/j.ultramic.2005.06.044

ABSTRACT In this paper, a shear force scanning near-field fluorescence microscope combined with a confocal laser microspectrofluorometer is described. The shear force detection is realized based on a bimorph cantilever, which provides a very sensitive, reliable, and easy to use method to control the probe–sample distance during scanning. With the system, high-quality shear force imaging of various samples has been carried out. Furthermore, simultaneous shear force and near-field fluorescence imaging of biological cells has also been realized. As an example, we especially present the result on the distribution of P-glycoprotein in the plasma membrane of human small cell lung cancer cells, suggesting that the system would be a promising tool for biological applications.

0 0
 · 
0 Bookmarks
 · 
18 Views
Data provided are for informational purposes only. Although carefully collected, accuracy cannot be guaranteed. The impact factor represents a rough estimation of the journal's impact factor and does not reflect the actual current impact factor. Publisher conditions are provided by RoMEO. Differing provisions from the publisher's actual policy or licence agreement may be applicable.

Keywords

bimorph cantilever
 
biological cells
 
confocal laser microspectrofluorometer
 
easy
 
human small cell lung cancer cells
 
near-field fluorescence imaging
 
plasma membrane
 
probe–sample distance
 
promising tool
 
scanning
 
shear force scanning near-field fluorescence microscope
 
various samples