Effect of dietary lipid level on growth performance, lipid deposition, hepatic lipogenesis in juvenile cobia (Rachycentron canadum). Aquaculture

Institute of Aquatic Economic Animals, School of Life Science, Sun Yat-sen University, 135 Xinggang West Road, Guangzhou 510275, PR China
Aquaculture (Impact Factor: 1.88). 09/2005; 249(1-4):439-447. DOI: 10.1016/j.aquaculture.2005.04.038


A study was undertaken to evaluate the effect of the dietary lipid level on growth, feed utilization, lipid deposition and lipid metabolism by cobia juveniles. Three isonitrogenous diets containing 47% crude protein with increasing dietary lipid levels 5%, 15% and 25% (DM, dry matter) were fed to satiety to triplicate groups of 20 fish (7.71 g) for 6 weeks. At the end of the feeding trial, fish fed diets containing 5% and 15% lipid showed a higher growth than those fish fed with 25% lipid. Though daily feed intake (DFI) decreased with increasing dietary lipid, there was no significant difference in daily energy intake (DEI) among treatments. As dietary lipid level increased, energy retention (EI), daily energy gain (DEG), daily lipid intake (DLI), daily lipid gain (DLG), viscerosomatic index (VSI), intraperitoneal fat ratio (IPF) and body lipid content increased dramatically and the 25% group had the highest values. Hepatosomatic index (HSI) and muscle lipid content were higher at 25% lipid group than 5% lipid group, but no significant different was found between 15% and 25% lipid group. Activities of G6PD and ME were reduced with increasing lipid intake, but activities of IDH and 6PGDH did not change among groups. In conclusion, high dietary lipid levels above 15% produced little practical benefit because of higher fat accretion in cobia.

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    • "In addition, in modern aquaculture HF diets have been widely used in many economic fish species to save dietary protein as an energy source and increase feed efficiency (Hillestad et al. 1998; Boujard et al. 2004). However, HF diets commonly caused excess fat accumulation in the liver or visceral fat tissue in farmed fish, accompanied by low growth, survival and resistance to pathogens and environmental stresses (Regost et al. 2001; Wang et al. 2005a), suggesting impaired lipid homeostasis. So far, people have known that the liver, adipose tissues, or muscle have the capability to store lipid in fish (Lin et al. 1977; Ando et al. 1993; Kaneko et al. 2013), and a number of lipid-metabolismrelated genes in some farmed fishes have been cloned and the preliminary functions have also been illustrated (He et al. 2014). "
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    ABSTRACT: Natural selection endows animals with the abilities to store lipid when food is abundant and to synthesize lipid when it is limited. However, the relevant adaptive strategy of lipid metabolism has not been clearly elucidated in fish. This study examined the systemic metabolic strategies of Nile tilapia to maintain lipid homeostasis when fed with low- or high-fat diets. Three diets with different lipid contents (1%, 7%, and 13%) were formulated and fed to tilapias for 10 weeks. At the end of the feeding trial, the growth rate, hepatic somatic index, and the triglyceride (TG) contents of serum, liver, muscle, and adipose tissue were comparable among three groups, whereas the total body lipid contents and the mass of adipose tissue increased with the increased dietary lipid levels. Overall quantitative PCR, western blotting and transcriptomic assays indicated that the liver was the primary responding organ to low-fat (LF) diet feeding, and the elevated glycolysis and accelerated biosynthesis of fatty acids (FA) in the liver is likely to be the main strategies of tilapia toward LF intake. In contrast, excess ingested lipid was preferentially stored in adipose tissue through increasing the capability of FA uptake and TG synthesis. Increasing numbers, but not enlarging size, of adipocytes may be the main strategy of Nile tilapia responding to continuous high-fat (HF) diet feeding. This is the first study illuminating the systemic adaptation of lipid metabolism responding to LF or HF diet in fish, and our results shed new light on fish physiology.
    08/2015; 3(8). DOI:10.14814/phy2.12485
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    • "However, fish can utilize dietary lipids up to a certain level beyond which a retarded growth may be achieved due to reduced feed consumption (Daniels and Robinson, 1986; Ellis and Reigh, 1991; Watanabe, 1982). Also, an excess amount of dietary lipid can result in some side effects including feed manufacturing problems, production of fatty liver, body lipid deposition, and lower carcass quality (Chatzifotis et al., 2010; López et al., 2006; Luo et al., 2005; Wang et al., 2005). Therefore, it is very important to optimize dietary protein and lipid levels for formulation of nutritionally balanced cost-effective practical diets of fish. "
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    ABSTRACT: An 8-week feeding trial was conducted to evaluate the effects of varying dietary protein and lipid levels on growth, feed utilization, and body composition of brown-marbled grouper (Epinephelus fuscoguttatus)and giant grouper (Epinephelus lanceolatus) hybrid. Eight diets were formulated to contain four protein levels (40, 45, 50 and 55% crude protein) and two lipid levels (7 and 14% crude lipid). Seven hundred and twenty juvenile grouper averaging 2.55 ± 0.10 g were randomly allotted to 24 cages (8 treatments in triplicate groups) and fed the test diets to apparent satiation three times a day. The results showed significant (P b 0.05) enhancement of weight gain and specific growth rate with increasing dietary protein from 40 to 50%, and a reduced growth was obtained by further increase of dietary protein to 55%. Increasing dietary lipid level from7 to 14% did not significantly affect growth performance (P N 0.05). Feed efficiency was significantly improved by increasing dietary protein and lipid levels. Also, the results revealed the significant increase of protein efficiency ratio by increasing dietary lipid. Significant reduction in daily feed intake was observed by increment of dietary protein and lipid levels. Whole-body lipid content increased with increasing dietary lipid and inversely correlated with whole-body moisture content. Dietary protein, lipid, and their interaction had a significant effect on liver protein content. Significant changes in dorsal muscle and liver fatty acid compositions were observed by variation of dietary protein and lipid levels. According to these results, a diet containing 50% protein and 14% lipid with protein to energy ratio of 23.9 mg kJ−1 is recommended for efficient growth of juvenile hybrid grouper.
    Aquaculture 05/2015; 446:283-289. DOI:10.1016/j.aquaculture.2015.05.019 · 1.88 Impact Factor
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    • "This has also been reported in juvenile cobia by Craig et al. (2006). In addition, Yamamoto et al. (2000), and Wang et al. (2005) reported lower feed consumption in rainbow trout and juvenile cobia fed diets with high energy, however, this was not the case in the present study, where feed intake values significantly increased with the increase of dietary lipid levels. "

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