Article

Application and Progress of Raman Tweezers in Single Cells

College of Physics and Electronic Engineering, Guangxi Normal University, Guilin 541004, China; Lab of Biophysics, Guangxi Academy of Sciences, Nanning 530003, China
Chinese Journal of Analytical Chemistry 01/2009; DOI: 10.1016/S1872-2040(08)60105-X

ABSTRACT Raman tweezers is a new optical technique that combines laser tweezers with Raman spectroscopy, which has the capability of studying single biological cells or organelles in nearly natured solution. Owing to its characteristics of noncontact, noninvasion, fast identification, and real-time detection, it has found application in improving the Signal Noise Ratio of Raman spectroscopy, optimizing the detection and sorting single cells or organelles, practicing the real-time detection of biochemistry dynamics process so as to make the activity and mechanisms of biomolecules understood deeply. The source, principle of Raman tweezers, and its optical application in biology are depicted and reviewed particularly in this article.

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