A 3D-impedimetric immunosensor based on foam Ni for detection of sulfate-reducing bacteria

Graduate School of the Chinese Academy of Sciences, 19 (Jia) Yuquan Road, Beijing 100039, China
Electrochemistry Communications (Impact Factor: 4.85). 02/2010; 12(2):288-291. DOI: 10.1016/j.elecom.2009.12.017


A 3D-immunosensor based on simple and efficient trapping platform (foam Ni) combining with adsorption of gold nanoparticles and specific recognition of biological/chemical molecular has been reported for detection of sulfate-reducing bacteria (SRB) using electrochemical impedance spectroscopy (EIS). The impedance spectra were also used to characterize the successful construct and stepwise modification of the impedimetric immunosensors. This results show that a linear relationship between electron-transfer resistance (Rct) values and the logarithm of the SRB concentrations was obtained for the SRB concentration range of 2.1 × 101–2.1 × 107 cfu/ml. Additionally, the fabricated immunosensor shows a high selectivity against other bacteria.

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    • "It is reported that the electrode made from macroporous silicon (3D) structure could be used as the efficient trapping platform for bacteria detection, and the obtained sensitivity was found to be more sensitive than a planar (2D) sensor. Wan et al. [28] developed a 3D-immunosensor based on antibody-functionalized 3D-foam Ni substrate as the trapping platform for detection of sulfate-reducing bacteria using impedimetric technique, which can detect the sulfate-reducing bacteria concentration range of 2.1 × 101–2.1 × 107 cfu·mL−1. "
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