A 3D-impedimetric immunosensor based on foam Ni for detection of sulfate-reducing bacteria
ABSTRACT A 3D-immunosensor based on simple and efficient trapping platform (foam Ni) combining with adsorption of gold nanoparticles and specific recognition of biological/chemical molecular has been reported for detection of sulfate-reducing bacteria (SRB) using electrochemical impedance spectroscopy (EIS). The impedance spectra were also used to characterize the successful construct and stepwise modification of the impedimetric immunosensors. This results show that a linear relationship between electron-transfer resistance (Rct) values and the logarithm of the SRB concentrations was obtained for the SRB concentration range of 2.1 × 101–2.1 × 107 cfu/ml. Additionally, the fabricated immunosensor shows a high selectivity against other bacteria.
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- "It is reported that the electrode made from macroporous silicon (3D) structure could be used as the efficient trapping platform for bacteria detection, and the obtained sensitivity was found to be more sensitive than a planar (2D) sensor. Wan et al.  developed a 3D-immunosensor based on antibody-functionalized 3D-foam Ni substrate as the trapping platform for detection of sulfate-reducing bacteria using impedimetric technique, which can detect the sulfate-reducing bacteria concentration range of 2.1 × 101–2.1 × 107 cfu·mL−1. "
ABSTRACT: The development of a rapid, sensitive, specific method for the foodborne pathogenic bacteria detection is of great importance to ensure food safety and security. In recent years impedimetric biosensors which integrate biological recognition technology and impedance have gained widespread application in the field of bacteria detection. This paper presents an overview on the progress and application of impedimetric biosensors for detection of foodborne pathogenic bacteria, particularly the new trends in the past few years, including the new specific bio-recognition elements such as bacteriophage and lectin, the use of nanomaterials and microfluidics techniques. The applications of these new materials or techniques have provided unprecedented opportunities for the development of high-performance impedance bacteria biosensors. The significant developments of impedimetric biosensors for bacteria detection in the last five years have been reviewed according to the classification of with or without specific bio-recognition element. In addition, some microfluidics systems, which were used in the construction of impedimetric biosensors to improve analytical performance, are introduced in this review.Sensors 12/2012; 12(3):3449-71. DOI:10.3390/s120303449 · 2.25 Impact Factor
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ABSTRACT: Mouse anti-human integrin β1 monoclonal antibody was assembled on glass carbon electrode by layer-by-layer adsorption. The determination of cervical cancer HeLa cells was performed using electrochemical impedance spectroscopy based on the immunoreaction between integrin β1 on cell membrane and the antibody immobilized on the electrode-surface. The factors influencing cell-detection, such as the concentration of gold nanoparticles in the modified film, the drying time of the modified film, the concentration of antibody and the binding time of antibody, were investigated, respectively. Under optimum conditions, the increasing extent of electrode-transfer resistance depended linearly on the cell concentration in the range of 1.0 × 104 to 2.0 × 106 cells mL−1 with a detection limit of 3.5 × 103 cells mL−1.Sensors and Actuators B Chemical 08/2010; 149(1-149):87-93. DOI:10.1016/j.snb.2010.06.026 · 4.10 Impact Factor
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ABSTRACT: A facile, sensitive and reliable impedimetric immunosensor doped with reduced graphene sheets (RGSs) and combined with a controllable electrodeposition technique was developed for the selective detection of marine pathogenic sulphate-reducing bacteria (SRB). The morphology of RGSs and the electrochemical properties of RGSs-doped chitosan (CS) nanocomposite film were investigated by atomic force microscopy, Fourier transform infrared spectroscopy, and cyclic voltammetry (CV). Electrochemical impedance spectroscopy and CV were used to verify the stepwise assembly of the sensor system. Faradic impedance spectroscopy for charge transfer for the redox probe Fe(CN)(6)(3-/4-) was done to determine SRB concentrations. The diameter of the Nyquist diagram that is equal to the charge-transfer resistance (R(ct)) increased with increasing SRB concentration. A linear relationship between R(ct) and SRB concentration was obtained in the SRB concentration range of 1.8×10(1) to 1.8×10(7) cfu/ml. The impedimetric biosensor gave a distinct response to SRB, but had no obvious response to Vibrio angillarum. It showed a high selectivity for the detection of the pathogen. Based on a combination of the biocompatibility of CS and good electrical conductivity of RGSs, a nanocomposite film with novel architecture was used to immobilize biological and chemical targets and to develop a new type of biosensor.Biosensors & Bioelectronics 08/2010; 26(5):1959-64. DOI:10.1016/j.bios.2010.08.008 · 6.41 Impact Factor