Determination of acetaldehyde in human blood by a gas chromatographie method with negligible artefactual acetaldehyde formation
ABSTRACT A method is described for determination of acetaldehyde in blood by head space gas chromatography.The method utilizes sodium nitrite-sulfosalicylic acid as an inhibitor of the ethanol oxidizing systems by means of which the interference of ethanol is reduced considerably. The detection limit was 0.4 μmol/l, the recovery 101.5 ± 5.2% and the coefficient of variation was 7.8% (1.5 μmol/l acetaldehyde). There was no disappearance of acetaldehyde if the head space vials were kept at −20°C for 24 h. In the comparison study with the semicarbazide method our results were 0.7–4.1 μmol/l lower. The values for acetaldehyde in blood after ethanol ingestion (0.5 g/kg) by volunteers were 0.5–1.3 μmol/l.
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ABSTRACT: This review is focused on the different chromatographic strategies for blood alcohol determination which can be adopted for clinical and/or forensic purposes. Particular attention is paid to gas chromatography and to high-performance liquid chromatography. However, other analytical techniques in common use, such as chemical and enzymic methods, are also briefly presented, together with some, at present unusual or experimental, approaches, such as enzymic reactors and catalytic electrodes, which are suitable for application in column liquid chromatography. Finally, mention is made of the methods for the determination of acetaldehyde, the major ethanol metabolite, and of some proposed markers of chronic alcohol abuse, such as acetaldehyde-protein adducts and carbohydrate-deficient transferrin. In order to give the background of knowledge for the rational choice of an analytical strategy, an updated outline of ethanol metabolism and toxicology is presented, together with basic information for the interpretation of the results. Problems concerning blood sampling and storage are also discussed.Journal of Chromatography A 10/1992; 580(1-2):161-90. · 4.26 Impact Factor
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ABSTRACT: The recently reported finding that a moderate alcohol consumption may lower the risk of cardiovascular disease prompted a study of the effects of ethanol and acetaldehyde on proliferation, viability and collagen secretion of rabbit aortic myocytes in culture and on the spontaneous efflux reaction of human platelets in vitro. Ethanol had no effects on any of the systems and acetaldehyde did not influence platelets significantly. Fifty μmol l-1 acetaldehyde diminished the proliferation and collagen secretion of arterial myocytes by 20% (P < 0·01) and 100 μmol l-1 acetaldehyde by 39% (P < 0·001) without affecting cell mass or cell death. A metabolic degradation, and some evaporation, of acetaldehyde was taking place and 50 μmol l-1 acetaldehyde was halved after approximately 2 h. The more ‘physiological’ concentration of acetaldehyde (5 μmol l-1) influenced cell proliferation significantly (P < 0·001) if the concentration was restored by 6-h intervals and the incubation time increased from 24 to 48 h. The weak aldehydedehydrogenase inhibitor chlorpropamide did not accentuate the effects of acetaldehyde.European Journal of Clinical Investigation 07/1984; 14(4):242 - 246. · 3.37 Impact Factor