A comparison of the TLC-densitometry and HPLC method for the determination of biogenic amines in fish and fishery products
ABSTRACT A comparative study on the suitability of chromatographic techniques such as thin layer chromatography (TLC)-densitometry and high performance liquid chromatography (HPLC) for the analysis of biogenic amines in fish and fishery products was carried out. The HPLC method was found to offer a good linearity (5–100 ng), sensitivity and repeatability (<3%), but required sophisticated instrumentation, technical skill and high operational cost and time. On the other hand, with a little loss in linearity (20–300 ng) and repeatability (<8%), TLC-densitometry was found to be rapid and less expensive. In addition, this method is suitable for rapid and simultaneous screening of several samples at a time. Therefore, the TLC-densitometric method can be effectively used in the fish industry to detect biogenic amines, especially the toxic histamine, and putrescine and cadaverine, which can potentiate histamine toxicity in fish and fishery products.
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ABSTRACT: Many plants, including strawflower and mistletoe, contain antioxidants and antimicrobials, which can increase the shelf life of seafood. The main aim of this study was to investigate the effect of mistletoe and strawflower extracts at doses of 0.5% (w/v) on the sensory, chemical and microbiological properties of rainbow trout fillets during 27 days of storage at 2 AE 1 °C. The phenolic compounds in these plants have been studied, but their effects on food quality and storage properties have not been reported. We found that extract of mistletoe did not extend the shelf life of the fillets; however, the strawflower extract show high antimicrobial activity in fish fillets. The shelf life of rainbow trout was 20 days for the control and fish treated with mistletoe extract and 23 days for fish treated with strawflower extract. The antioxidant effect of extracts on fish fillets was weak, whereas strawflower extract had high antimicrobial effect. Perox-ide value (PV) and thiobarbituric acid (TBA) values fluctuated during storage periods around main values below 14 meq O 2 kg À1 and 0.6 MA kg À1 , respectively. Putrescine, cadaverine, spermine, spermidine, sero-tonin, tyramine and dopamine were main amine, whereas histamine accumulated at low levels (<2 mg per 100 g). Strawflower suppressed biogenic amine accumulation in fish fillets. At the limit of acceptability, total viable count (TVC), Enterobacteriaceae and lactic acid bacteria count remained below 7.6, 6.83 and 8.01 log CFU g À1 , respectively. The results of this study show that ethanol extracts of strawflower improve the shelf life of rainbow trout.
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ABSTRACT: In order to obtain the level of biogenic amines in fish and fish products, a reversed phase high performance liquid chromatographic method was used for the quantitation of 12 biogenic amines in 300 fish and 80 fish products in Korea. Biogenic amines were extracted with hydrochloric acid, derivatized with dansyl chloride, separated using gradient elution, and detected by photodiode array and all detected biogenic amines were identified by UPLC/MS/MS. The detection levels of biogenic amines in the sample ranged from ND (not detected) to 30.6mg/kg for agmatine, from ND to 390.0mg/kg for cadaverine, from ND to 19.8mg/kg for dopamine, from ND to 70.1mg/kg for histamine, from ND to 17.8mg/kg for noradrenaline, from ND to 29.7mg/kg for 2-phenylethylamine, from ND to 43.0mg/kg for putrescine, from ND to 11.9mg/kg for serotonin, from ND to 9.5mg/kg for spermidine, from ND to 13.5mg/kg for spermine, from ND to 12.8mg/kg for tryptamine, and from ND to 67.7mg/kg for tyramine. Anchovy samples showed the high levels of histamine from 15.2 to 70.1mg/kg, putrescine from 9.1 to 43.0mg/kg, cadaverine from 152.2 to 90.0mg/kg, and tyramine from 27.1 to 67.7mg/kg. Agmatine was found at 30.6mg/kg in cuttlefish. In the others fish samples agmatine detection levels were in the range from ND to 27.2mg/kg. Spermidine and dopamine were detected about more than 85% samples in the range from ND to 19.3mg/kg. Most of fish product samples showed less than 20mg/kg of each biogenic amine. The maximum histamine and tyramine detection levels were respectively less than those of the regulation and recommendation.Food Control. 01/2010; 21(9):1219-1226.
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ABSTRACT: We report an investigation of the structural properties of CuCr0.95Mg0.05O2 films on c-plane sapphire substrates using pulsed laser deposition. The thin films were grown at different temperatures of 500, 600, and 700°C with an oxygen partial pressure of 10mTorr. c-axis oriented epitaxial CuCr0.95Mg0.05O2 thin films on c-plane sapphire substrates with an in-plan 30° rotation were obtained. The sixfold rotational symmetry in the pole figures from the (012) plane indicates that there are two different types of crystal grains in which the a-axes rotate by 60° with respect to each other around the c-axis. The reason for the 30° rotation is assumed to be the presence of the ∼10% mismatch of oxygen distance between the c-plane sapphire substrate and the CuCr0.95Mg0.05O2 on the c-plane. The epitaxial crystallographic relationship between CuCr0.95Mg0.05O2 and Al2O3 was (0006)CuCrO2//(0003)Al2O3 and [10−10]CuCrO2//[11−20]Al2O3. The presence of twins in the films and the surface morphology were investigated using transmission electron microscopy and scanning electron microscopy, respectively.Journal of Crystal Growth 07/2011; 326(1):9-13. · 1.55 Impact Factor