Article

Isolation of lysosomes from the tail of metamorphosing bullfrog tadpole

Life Science Institute, Sophia University, Chiyoda-ku, Tokyo 102, Japan
Comparative Biochemistry and Physiology Part B: Comparative Biochemistry DOI:10.1016/0305-0491(85)90373-6 pp.603-607

ABSTRACT 1.1. A mitochondrial lysosomal fraction obtained by differential centrifugation of the tail homogenate of metamorphosing Rana catesbeiana tadpoles was fractionated on iso-osmotic self generating gradients composed of modified colloidal silica (Percoll).2.2. More than half the activities of most lysosomal hydrolases were recovered to a heavy fraction, which contained little activities of cytochrome oxidase and of catalase.3.3. The activities of lysosomal enzymes in the heavy fraction showed latency, and the median density of those was 1.09.4.4. These results indicate that intact lysosomes, which are considerably purified, could be obtained by this method.

0 0
 · 
0 Bookmarks
 · 
15 Views
Data provided are for informational purposes only. Although carefully collected, accuracy cannot be guaranteed. The impact factor represents a rough estimation of the journal's impact factor and does not reflect the actual current impact factor. Publisher conditions are provided by RoMEO. Differing provisions from the publisher's actual policy or licence agreement may be applicable.

Keywords

differential centrifugation
 
iso-osmotic self
 
latency
 
lysosomal enzymes
 
lysosomal hydrolases
 
metamorphosing Rana catesbeiana tadpoles
 
mitochondrial lysosomal fraction
 
tail homogenate