Article

Inhibition of N-acetyltransferase activity and gene expression in human colon cancer cell lines by diallyl sulfide

Department of Microbiology, China Medical University, No 91, Hsueh-Shih road, Taichung 400, Taiwan, Republic of China; Department of Clinical Pathology, Chen Hsin Rehabitation Medical Center, Taipei, Taiwan, ROC; Department of Urology, China Medical University Hospital, Taichung 400, Taiwan, ROC; Department of Surgery, Jen-Ai Hospital, 483, Tong-Rong Road, Tali, Taichung 400, Taiwan, ROC; Department of Radiological Technology, Chung Tai Institute of Health Sciences and Technology, Taichung 400, Taiwan, ROC; Department of Surgery, China Medical University Hospital, Taichung 400, Taiwan, ROC
Food and Chemical Toxicology DOI:10.1016/j.fct.2003.08.015 pp.195-202

ABSTRACT Diallyl sulfide (DAS) is one of the major components of garlic (Allium sativum) and is widely used in the world for food. In this study, DAS was selected for testing the inhibition of arylamine N-acetyltransferase (NAT) activity (N-acetylation of 2-aminofluorene) and gene expresseion (mRNA NAT) in human colon cancer cell lines (colo 205, colo 320 DM and colo 320 HSR). The NAT activity was examined by high preformance liquid chromatography and indicated that a 24 h DAS treatment decreases N-acetylation of 2-aminofluorene in three colon (colo 205, 320 DM and colo 320 HSR) cancer cell lines. The NAT enzymes (protein) were analyzed by western blotting and flow cytometry and it indicated that DAS decreased the levels of NAT in three colon (colo 205, 320 DM and colo 320 HSR) cancer cell lines. The gene expression of NAT (mRNAT NAT) was determined by polymerase chain reaction (PCR), it was shown that DAS affect mRNA NAT expression in examined human colon cancer cell lines. This report is the first to demonstrate that DAS does inhibit human colon cancer cell NAT activity and gene expression.

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Keywords

24 h DAS treatment decreases N-acetylation
 
arylamine N-acetyltransferase
 
DAS
 
Diallyl sulfide
 
gene expresseion
 
gene expression
 
human colon cancer cell lines
 
human colon cancer cell NAT activity
 
major components
 
mRNA NAT
 
mRNA NAT expression
 
mRNAT NAT
 
NAT
 
NAT activity
 
NAT enzymes
 
polymerase chain reaction
 
preformance liquid chromatography
 
western blotting