The detection of antibodies to Mycobacterium tuberculosis by microplate enzyme linked immunosorbent assay (ELISA)

Department of Pathology, Harefield Hospital, Harefield, Middlesex, UK
Tubercle 03/1976; 57(1):67-70. DOI: 10.1016/0041-3879(76)90019-2


The detection of antibodies to Mycobacterium tuberculosis by enzyme-linked immunosorbent assay has proved to be a potentially useful technique for the serodiagnosis of tuberculosis. The technique is capable of full automation. The use of a purified antigen should further improve the sensitivity of the method.RésuméLa mise en évidence d'anticorps vis-à-vis de Mycobacterium tuberculosis dans l'étude d'un immunosorbent lié à des enzymes s'est révelée une technique qui pourrait être utile dans le serodiagnostic de la tuberculose. Cette technique peut être entièrement automatisée. L'utilisation d'un antigène purifié devrait améliorer encore la sensibilité de la méthode.ResumenLa detection de anticuerpos del Mycobacterium tuberculosis por medio de immunoabsorbentes ligados a enzimas ha demostrado ser una técnica potencialmente útil para el sero-diagnóstico de la tuberculosis. La técnica puede ser totalmente automática. El use de un antígeno purificado puede aun mejorar la sensibilidad del méthoo.

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    • "A serum dilution was considered positive if it yielded a mean OD of each group equal to, or greater than cut off value according to (Dimitri et al., 1987). Cut off value was calculated according to (Nassau et al., 1976), which equal to the mean OD of negative serum plus 2 standard deviation. "
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    ABSTRACT: Bovine tuberculosis represents one of the very important infectious diseases in Egypt and the world. It has zoonotic importance and causes severe economic losses. Accurate and rapid diagnosis considered as the milestone for control of the disease. In this study ELISA technique was used for confirmation of positive reactors cows that tested with single intradermal tuberculin test, to detect false positive reactors. Bovine PPD and ST.CF antigens have been used as two different coating antigens for ELISA technique. 3747 cattle from dairy farms in five different governorates were subjected to the single intradermal cervical tuberculin test whereas 78 (2.24%) proved positive reactors to tuberculin. These positive reactors tested with ELISA. 64 (82.05%) animals were positive by ELISA coated with ST-CF, while by using bovine PPD as coating antigen 58 (74.35%) animals were positive. The previous results indicated that ELISA test showed higher sensitivity and specificity using ST-CF as coating antigen than in case of bovine PPD coating antigen.
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    • "The heavy global public health burden of TB worldwide demands for the development of more rapid and sensitive detection methods. To date, many methods and techniques have been developed for rapid detection of M. tuberculosis, such as polymerase chain reaction (PCR) [6– 9], latex agglutination [10], enzyme-linked immunosorbent assay (ELISA) [11] [12] [13], radiometric detection [14], genprobe amplified M. Tuberculosis direct test (AMTDT) [15], TB rapid cultivation detection technique, such as MB/Bact system, BactecMGIT 960 system [16] [17] and flow cytometry [18]. These methods are more sensitive and rapid than the traditional microbial culture-based methods, as summarized in Table 1. "
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    ABSTRACT: Biosensing technologies promise to improve Mycobacterium tuberculosis (M. tuberculosis) detection and management in clinical diagnosis, food analysis, bioprocess, and environmental monitoring. A variety of portable, rapid, and sensitive biosensors with immediate "on-the-spot" interpretation have been developed for M. tuberculosis detection based on different biological elements recognition systems and basic signal transducer principles. Here, we present a synopsis of current developments of biosensing technologies for M. tuberculosis detection, which are classified on the basis of basic signal transducer principles, including piezoelectric quartz crystal biosensors, electrochemical biosensors, and magnetoelastic biosensors. Special attention is paid to the methods for improving the framework and analytical parameters of the biosensors, including sensitivity and analysis time as well as automation of analysis procedures. Challenges and perspectives of biosensing technologies development for M. tuberculosis detection are also discussed in the final part of this paper.
    Clinical and Developmental Immunology 03/2011; 2011(1):193963. DOI:10.1155/2011/193963 · 2.93 Impact Factor
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    • "Methods for diagnosing Mycobacterium. tuberculosis (M.TB) include latex agglutination [4] and ELISA [5] [6], the radiometric detection [7], the bacteriological examination , DNA [8 Á/10] and RNA [11] probes, polymerase chain reaction (PCR) [12 Á/14], TB "
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    ABSTRACT: The bulk acoustic wave impedance biosensor was set up and used to monitor the growth of Mycobacterium tuberculosis (M.TB). This sensor is rapid, simple, sensitive (lower limit is 2 x 10(3) cells ml(-1)) and cheap (easy to generalize). The typical response curve was different from other bacteria's, such as Escherichia coli, Staphylococcus aureus, Proteus mirabilis. The frequency detection time was used to quantitatively determine M.TB. It was proportional to logarithm of the initial concentration of M.TB in the range of 2 x 10(3)-3 x 10(7) cells ml(-1). The set up sensor was applied to the direct diagnosis of M.TB samples. The interference of other bacteria was eliminated by pretreatment. Our results confirmed that the use of the set up biosensor was reliable, sensitive. It gives out the potential use for determining M.TB.
    Talanta 05/2003; 59(5):935-41. DOI:10.1016/S0039-9140(02)00643-4 · 3.55 Impact Factor
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