Amperometric biosensor based on carbon nanotubes coated with polyaniline/dendrimer-encapsulated Pt nanoparticles for glucose detection
ABSTRACT A novel amperometric glucose biosensor based on the nanocomposites of multi-wall carbon nanotubes (CNT) coated with polyaniline (PANI) and dendrimer-encapsulated Pt nanoparticles (Pt-DENs) is prepared. CNT coated with protonated PANI is in situ synthesized and Pt-DENs is absorbed on PANI/CNT composite surface by self-assembly method. Then Glucose oxidase (GOx) is crosslink-immobilizated onto Pt-DENs/PANI/CNT composite film. The results show that the fabricated GOx/Pt-DENs/PANI/CNT electrode exhibits excellent response performance to glucose, such as low detection limit (0.5 µM), wide linear range (1 µM–12 mM), short response time (about 5 s), high sensitivity (42.0 µA mM− 1 cm− 2) and stability (83% remains after 3 weeks).
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ABSTRACT: A novel glucose biosensor was fabricated by integrating glucose oxidase (GOx) with chitosan/NiFe2O4 nanoparticles (CHIT/NiFe2O4NPs) on a glassy carbon electrode (GCE). The properties of CHIT/NiFe2O4NPs/GOx were characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM), UV–vis spectroscopy, Fourier transform infrared spectroscopy (FTIR) and electrochemical impedance spectroscopy (EIS). The GCE modified with the CHIT/NiFe2O4NPs/GOx showed excellent electrocatalytical response to the oxidation of glucose when ferrocene carboxylic acid was used as an artificial redox mediator, which was studied by cyclic voltammetry (CV). Different parameters including GOx concentration, working potential and pH of supporting electrolyte that governed the analytical performance of the biosensor, have been studied in detail and optimized. The biosensor was applied to detect glucose with a linear range of 1 × 10−4–2.0 × 10−2 mol L−1. The biosensor exhibited excellent performance for glucose at applied potential of 0.6 V with a fast response time (<4 s).Sensors and Actuators B Chemical 03/2010; 145(1-145):293-298. DOI:10.1016/j.snb.2009.12.018 · 3.84 Impact Factor
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ABSTRACT: In this paper a highly sensitive glucose biosensor is proposed based on a polysilicon (poly-Si) wire structure coated with 3-aminopropyltriethoxysilane (γ-APTES) mixed with polydimethylsiloxane-treated hydrophobic fumed silica nanoparticles (NPs) as the sensing membrane. The γ-APTES and fumed silica NPs mixture was directly transferred to and coated onto the poly-Si wire region with the help of a focus-ion-beam (FIB) processed capillary atomic-force-microscope (C-AFM) tip. After the necessary curing and UV illumination processes, the resultant sensor showed an extremely wide linear detection range from 0.1μM to 10mM with a channel current sensitivity as high as 5.33AmM−1cm−2 (or a channel conductance sensitivity of 70μSmM−1), and a detection limit as low as 10nM can be achieved. Our experimental results showed that the poly-Si wire sensor has good selective analysis and operational stability on glucose detection under a 10:1 concentration ratio of glucose and uric acid. Its linear range and lowest detection limit remain virtually unimpaired in the presence of uric acid.Sensors and Actuators B Chemical 10/2009; 142(1):273-279. DOI:10.1016/j.snb.2009.08.003 · 3.84 Impact Factor
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ABSTRACT: A new amperometric glucose biosensor has been developed based on platinum (Pt) nanoparticles/polymerized ionic liquid-carbon nanotubes (CNTs) nanocomposites (PtNPs/PIL-CNTs). The CNTs was functionalized with polymerized ionic liquid (PIL) through directly polymerization of the ionic liquid, 1-vinyl-3-ethylimidazolium tetrafluoroborate ([VEIM]BF4), on carbon nanotubes and then used as the support for the highly dispersed Pt nanoparticles. The electrochemical performance of the PtNPs/PIL-CNTs modified glassy carbon (PtNPs/PIL-CNTs/GC) electrode has been investigated by typical electrochemical methods. The PtNPs/PIL-CNTs/GC electrode shows high electrocatalytic activity towards the oxidation of hydrogen peroxide. Taking glucose oxidase (GOD) as the model, the resulting amperometric glucose biosensor shows good analytical characteristics, such as a high sensitivity (28.28 μA mM−1 cm−2), wide linear range (up to 12 mM) and low detection limit (10 μM).Electrochimica Acta 03/2010; 55(8-55):2848-2852. DOI:10.1016/j.electacta.2009.12.057 · 4.09 Impact Factor