Article

Salt tolerance of genetic modified potato (solanum tuberosum) cv. Agria by expression of a bacterial mtlD gene

Advances in Agriculture & Botanics - Bioflux 01/2012;

ABSTRACT Water and salinity stresses are the major reasons to yield decreasing in the world. Potato is the world’s
main tuber crops of the Solanaceae family which is one of the most economically important annual vegetable crop.
The goal of this investigation was creation genetic modified potato cv.Agria with more tolerance to salinity stress
and evaluating GMO potato properties. To create transgenic potato plant, mtlD gene (mannitol -1- phosphate
dehydrogenase, E.C.1.1.1.17) was expressed to potato cv.Agria plant by using Agrobacterium tumefaciens.
Transgenic potato was produced by transforming of mtlD gene to potato plant cv.Agria. Existence of recombinant
gene in transgenic plants was approved by two ways 1. Polymerase Chain Reaction technique. 2. Measurement of
physiological parameters. The transgenic potatoes and non-transgenic potatoes lines exhibited the different
amounts of tolerance to salinity stress because in the transgenic lines mannitol accumulates that increased osmotic
pressure in salinity stress. The salt tolerance of transgenic potato cv.Agria (+mtlD) was recorded higher than that of
non-transgenic potato cv.Agria (-mtlD). Osmotic pressure in this transgenic potato plant was increased by
accumulating of mannitol and existence of mannitol in potato plant approved that the mtlD gene was successfully
expressed to potato cv.Agria.

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Available from: Ali Parsaeimehr, Jul 07, 2015
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