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Salt tolerance of genetic modified potato (solanum tuberosum) cv. Agria by expression of a bacterial mtlD gene

Advances in Agriculture & Botanics - Bioflux 01/2012;

ABSTRACT Water and salinity stresses are the major reasons to yield decreasing in the world. Potato is the world’s
main tuber crops of the Solanaceae family which is one of the most economically important annual vegetable crop.
The goal of this investigation was creation genetic modified potato cv.Agria with more tolerance to salinity stress
and evaluating GMO potato properties. To create transgenic potato plant, mtlD gene (mannitol -1- phosphate
dehydrogenase, E.C.1.1.1.17) was expressed to potato cv.Agria plant by using Agrobacterium tumefaciens.
Transgenic potato was produced by transforming of mtlD gene to potato plant cv.Agria. Existence of recombinant
gene in transgenic plants was approved by two ways 1. Polymerase Chain Reaction technique. 2. Measurement of
physiological parameters. The transgenic potatoes and non-transgenic potatoes lines exhibited the different
amounts of tolerance to salinity stress because in the transgenic lines mannitol accumulates that increased osmotic
pressure in salinity stress. The salt tolerance of transgenic potato cv.Agria (+mtlD) was recorded higher than that of
non-transgenic potato cv.Agria (-mtlD). Osmotic pressure in this transgenic potato plant was increased by
accumulating of mannitol and existence of mannitol in potato plant approved that the mtlD gene was successfully
expressed to potato cv.Agria.

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    ABSTRACT: Previous work on a number of transgenics having mtlD has esablished the role of mannitol accumulation in the alleviation of abiotic stresses like salinity and drought. In the present study we have characterized the peanut (cv. GG 20) plants transformed with mtlD (from Escherichia coli) for its tolerance to abiotic stresses. Salinity and water-deficit stress tolerance were evaluated using different physio-biochemical and growth parameters in transgenic and wild-type plants both at seedling and full-growth stage. Here we demonstrate that biosynthesis of mannitol in transgenic peanut lines due to the over-expression of mtlD gene improves its tolerance for salinity and water-deficit stress over WT. This was revealed by better growth and physio-biochemical parameters like mannitol content, proline levels, total chlorophyll content, osmotic potential, electrolytic leakage and relative water content in transgenics over WT. It is concluded that the better performance of mannitol-synthesizing transgenic plants was due to the stress-shielding role of mannitol. However we are not ruling out the possibility of induction of a series of signal- transductions in transgenic plants in response to the mtlD expression, which may activate other protective reactions against salinity and drought stresses.
    Australian Journal of Crop Science 03/2014; 8(3):413. · 1.63 Impact Factor

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