Article

FAAS determination of metal nutrients in fish feed after ultrasound extraction

Faculdade de Medicina Veterinária e Zootecnia, Programa de Pós-Graduação em Zootecnia, Departamento de Melhoramento e Nutrição Animal – UNESP, Caixa Postal 560, 18618-000 Botucatu, SP, Brazil; Instituto de Biociências, Departamento de Química e Bioquímica – UNESP, Caixa Postal 510, 18618-000 Botucatu, SP, Brazil; Instituto de Biociências, Departamento de Física e Biofísica – UNESP, Caixa Postal 510, 18618-000 Botucatu, SP, Brazil
Food Chemistry 01/2009; DOI: 10.1016/j.foodchem.2008.07.070

ABSTRACT This paper proposes a method to determine calcium, magnesium, manganese and zinc in fish feed samples using ultrasound in the analyte extraction process, and subsequent quantification by flame atomic absorption spectrometry (FAAS). Using 0.10 mol/L of HCl as extraction solution, the optimal conditions of extraction were established as follows: 100 mg of sample mass; sample granulometry of less than 60 μm; sonication time of three cycles of 10 s and sonication power of 102 W. The method was applied in studies of the digestibility of these nutrients in samples of fish feed used in the diet of Nile tilapia juveniles. The results achieved were congruent with those obtained from the mineralization of fish feed samples in the metal nutrient extraction process.

1 Bookmark
 · 
216 Views

Full-text

View
150 Downloads
Available from
May 17, 2014