Fermentative hydrogen production by a new chemoheterotrophic bacterium Rhodopseudomonas Palustris P4

Department of Food Science and Technology, Kyungsung University, Daeyeun-dong, Nam-ku, Pusan 608-736, South Korea
International Journal of Hydrogen Energy (Impact Factor: 2.93). 11/2002; 27(11):1373-1379. DOI: 10.1016/S0360-3199(02)00100-3

ABSTRACT A newly isolated Rhodopseudomonas palustris P4 for CO-dependent H2 production was studied for its capability of fermentative H2 production in batch cultivations. Important parameters investigated include pH, temperature, concentrations of phosphate and glucose, intermittent purging of culture broth by argon gas, and kind of sugars. The pH of the culture medium significantly decreased as fermentation proceeded due to the accumulation of various organic acids, and this inhibited the H2 production seriously. The use of fortified phosphate at 60– could alleviate this inhibition. The increase of glucose concentration (1–) resulted in higher H2 production, but the yield of H2 production (mmol H2/mmol glucose) gradually decreased with increasing glucose concentration. Intermittent purging of the culture broth by argon gas improved H2 production. Carbon mass balance showed that, in addition to cell mass, ethanol, acetate and CO2 were the major fermentation products that comprised more than 70% of carbon consumed. R. palustris P4 could utilize various monosaccharides (glucose, galactose, fructose), disaccharides (lactose, sucrose) and starch to produce H2. However, the H2 production rate with disaccharides and starch was much slower than that with monosaccharides. The maximal H2 yield and H2 production rate were estimated to be H2/mmol glucose and H2/g cell h, respectively. These results indicate that, although isolated for CO-dependent H2 production, R. palustris P4 has a high potential as a fermentative H2 producer.

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    • "The key points for improving the Y H are also advancements in hydrodynamic aspects, bioreactor design, gas separation, light intensity and its distribution inside culture thickness [8]. Over the years, many scientists investigated the photofermentation process using nonsulfur photosynthetic bacteria for indoor hydrogen photoproduction under batch growth conditions [9] [10] [11]. "
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    ABSTRACT: The main goal of this study was to increase the hydrogen production rate improving the culture technique and the photobioreactor performances. Experiments were carried out at a constant culture temperature of 30°C and at an average irradiance of 480 W m(-2) using a cylindrical photobioreactor (4.0 cm, internal diameter). The culture technique, namely, the semicontinuous regime for growing Rhodopseudomonas palustris 42OL made it possible to achieve a very high daily hydrogen production rate of 594 ± 61 mL (H(2)) L(-1) d(-1). This value, never reported for this strain, corresponds to about 25 mL (H(2)) L(-1) h(-1), and it was obtained when the hydraulic retention time (HRT) was of 225 hours. Under the same growth conditions, a very high biomass production rate (496 ± 45 mg (dw) L(-1) d(-1)) was also achieved. Higher or lower HRTs caused a reduction in both the hydrogen and the biomass production rates. The malic-acid removal efficiency (MA(re)) was always higher than 90%. The maximal hydrogen yield was 3.03 mol H(2) mol MA(-1) at the HRT of 360 hours. The highest total energy conversion efficiency was achieved at the HRT of 225 hours.
    BioMed Research International 07/2012; 2012:590693. DOI:10.1155/2012/590693 · 2.71 Impact Factor
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    • "Among the photosynthetic microorganisms, photosynthetic bacteria exhibit a high level of hydrogen production. Nonetheless, low conversion efficiencies of other biological systems can be compensated for, by low energy requirements and reduced initial investment costs (Macler et al., 1979; Oh et al., 2002). Moreover, in laboratory experiments, high light energy conversion efficiency upto 7% has been reported for photoheterotrophic processes (Nakada et al., 1995; Sunita & Mitra, 1993; Ueno et al., 1995)It is reported by Rupprecht et al. (2006) that continuously depleting oil reserves had necessitated for search of alternative energy sources. of clean fuels and less CO 2 emission to reduce the impact of global warming is meet by employing potoheterotrophic and photoautotrophic organisms in bio-H 2 production process. "
    Biofuel Production-Recent Developments and Prospects, 09/2011; , ISBN: 978-953-307-478-8
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    • "Previous studies have been shown that hydrogen gas can be produced by using pure cultures of anaerobic bacteria such as Clostridium acetobutyricum [9]. However, there are few reports using pure strains of Clostridium sp. to produce hydrogen because of the difficulty in cultivating strictly anaerobic strains [10]. Recently, many researchers have focused on the hydrogen producing ability of microbial strains isolated from waste materials, for instance, cow dung compost [11], leachate [12] and sewage sludge [13] [14]. "
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    ABSTRACT: We isolated hydrogen producing microbial strains from municipal sewage sludge in Thailand. The experiments were performed in 0.7 liter of Reinforced Clostridial Media (RCM) at pH 6.8 and 301C. Hydrogen producing isolates were identified as Escherichia coli and Enterobacter sp. by 16S ribosomal DNA sequence analysis. E. coli S3 demonstrated a maximum hydrogen production rate of 16.070.53 ml-H 2 /l/h after 24 hours of incubation. The maximal cumulative hydrogen yield of 269.928.87 ml (calculated at STP) and the hydrogen yield of 0.840.06 mol-H 2 /mol-glucose were obtained at an initial glucose concentration of 0.5% (w/v), pH 6.8 and 301C.
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