Antibiotic resistance in Gram-negative bacteria from cultured catfish and aquaculture ponds
ABSTRACT The incidence of antibiotic resistance was compared in Gram-negative bacteria isolated from the intestinal tracts of catfish and from water and sediment in aquaculture ponds and rivers of the southeastern United States. Resistance to tetracycline, oxytetracycline, chloramphenicol, kanamycin, ampicillin, and nitrofurantoin was determined. The predominating microflora were Plesiomonas shigelloides and Aeromonas hydrophila. Individual and multiple antibiotic resistances were associated with antimicrobial use. Resistance apparently was higher in ponds undergoing antimicrobial therapy or with a history of recent treatment than in ponds without recent antimicrobial treatment. The lowest incidence of resistance was found in riverine bacteria.
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ABSTRACT: Two experiments were simultaneously conducted with Morus alba (white mulberry) foliage extract (MFE) as a growth promoter and treatment of Aeromonas hydrophila infection in separate 60 and 30 days trail (Experiments 1 and 2, resp.) in African catfish (Clarias gariepinus). In Experiment 1, four diets, control and control supplemented with 2, 5, or 7 g MFE/kg dry matter (DM) of diet, were used. In Experiment 2, fish were intraperitoneally infected with Aeromonas hydrophila and fed the same diets as experiment 1 plus additional two diets with or without antibiotic. Results of experiment 1 showed that growth was unaffected by dietary levels of MFE. Treatments with the inclusion of MFE at the levels of 5 and 7 g/Kg DM had no mortality. Red blood cells (RBC), albumin, and total protein were all higher for the treatments fed MFE (5 and 7 g/Kg DM). Results of experiment 2 showed RBC, hemoglobin, hematocrit, globulin, albumin, and total protein improved with the increase in MFE in the infected fish. The dietary MFE at the level of 7 g/kg DM reduced mortality rate. In conclusion, MFE at the level of 7 g/kg DM could be a valuable dietary supplement to cure the infected fish.12/2014; 2014:e592709. DOI:10.1155/2014/592709
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ABSTRACT: Introduction Giant freshwater prawn (Macrobrachium rosenbergii (de Man, 1879) (or scampi) is an important commercial species due to property as food supply as well as a valuable export product. In India, giant freshwater prawn distributes mainly in the Southern region where environmental conditions are most favorable for the ISSN: 2347-3215 Volume 1 Number 1 (2013) pp. 01-16 The giant freshwater prawn Macrobrachium rosenbergii, popularly known as scampi, farmed crustacean species was used in this study. Fresh water prawn samples collected in two places. First place, Fresh water Prawn hatchery area, Marakanam and Neelakarai, this sample should be considered as a set=1. Second place, samples from various market areas in and around Kancheepuram town, Tamilnadu, this sample should be considered as set=2. The correlation between Total coliform Count (TCC) and Total Bacterial Count (TBC) of Set 1 and 2 was considerably significant statistically (r=0.40; p<0.01 and r=0.55; p<0.01). The positive regression line in the present study also supports that the increase in TCC with the increase in TBC. The principal bacterial genera encountered in fresh water prawn comprised of E. coli, Pseudomonas sps, Enterobacter sps, Vibrio sps Aeromonas sps and Staphylococcus aureus. Proteolytic activity and lipolytic activity were also found in the bacterial isolates which were isolated from giant fresh water prawn (Macrobrachium rosenbergii) digestive system. The antibiotic sensitivity showed Oxytetracycline antibiotic was highly sensitive to most of the bacterial isolates from the prawn samples. Hence, Knowledge of the qualitative and quantitative aspects of bacterial flora in the hatchery would help to understand disturbances, if any, brought about during disease outbreaks. .