Article

Molecular fingerprinting of Staphylococcus aureus isolated from patients with osteomyelitis in Argentina and clonal distribution of the cap5(8) genes and of other selected virulence genes.

Departamento de Microbiología, Parasitología e Inmunología, Facultad de Medicina, Universidad de Buenos Aires, Paraguay 2155 P-12, C1121ABG, Buenos Aires, Argentina.
European Journal of Clinical Microbiology (impact factor: 2.86). 03/2012; 31(10):2559-66. DOI:10.1007/s10096-012-1596-8 pp.2559-66
Source: PubMed

ABSTRACT The molecular fingerprinting of a collection of 94 Staphylococcus aureus isolates from patients with osteomyelitis in Argentina was performed. Twenty-three SmaI pulsed-field gel electrophoresis (PFGE) types and 37 spa types were identified. The isolates were assigned to 23 sequence types (STs). The proportion of methicillin-resistant S. aureus (MRSA) isolates was significantly higher among cap5 S. aureus (35/61) compared with cap8 S. aureus (8/33) isolates (p = 0.0025). Twenty-four of the 94 isolates carried the lukS-PV/lukF-PV genes, which were significantly associated to cap5 [(23/38) compared with cap8 S. aureus isolates (1/32) (p = 0.0001)]. Forty of the 94 isolates carried genes of the egc locus (seg/sei). The distribution of seg/sei genes among isolates was related to certain clones. Isolates of the four agr types were found in the S. aureus collection. Whereas agr I isolates were evenly distributed among cap5 and cap8 S. aureus isolates (32/61 and 14/33, respectively), the agr II group was composed of 29 cap5 S. aureus isolates and agr III was composed of 16 cap8 S. aureus isolates. Two clones originally associated to animals (ST 188, 7 isolates and ST 1796, 5 isolates) were associated with chronic osteomyelitis and lack of capsular polysaccharide (CP) production. Loss of CP production remains the single factor among those investigated that is associated with chronic osteomyelitis.

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  • Article: Aromatic Compound-Dependent Staphylococcus aureus Is Safe in a Nasal Colonization Leukopenic Murine Model.
    María S Barbagelata, Lucía P Alvarez, Cristian M Dotto, Santiago M Lattar, Daniel O Sordelli, Fernanda R Buzzola
    [show abstract] [hide abstract]
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Keywords

23 sequence types
 
37 spa types
 
94 Staphylococcus aureus
 
agr II group
 
agr III
 
cap5 S. aureus
 
cap8 S. aureus
 
capsular polysaccharide
 
certain clones
 
chronic osteomyelitis
 
egc locus
 
genes
 
lukS-PV/lukF-PV genes
 
methicillin-resistant S. aureus
 
molecular fingerprinting
 
MRSA
 
S. aureus collection
 
seg/sei genes
 
single factor
 
SmaI pulsed-field gel electrophoresis