Topical Application of Chrysanthemum indicum L. Attenuates the Development of Atopic Dermatitis-Like Skin Lesions by Suppressing Serum IgE Levels, IFN-γ, and IL-4 in Nc/Nga Mice

Department of Food and Nutrition, Hoseo University, 165 Sechul-Ri, BaeBang-Yup, Asan-Si, ChungNam-Do 336-795, Republic of Korea.
Evidence-based Complementary and Alternative Medicine (Impact Factor: 2.18). 02/2012; 2012:821967. DOI: 10.1155/2012/821967
Source: PubMed

ABSTRACT Chrysanthemum indicum L. (CIL) is widely used as an anti-inflammatory agent in Asia and our preliminary study revealed that CIL reduced interleukin (IL)-4 and IL-13 in 2,4-dinitrochlorobenzene (DNCB)-treated HaCaT cells, a human keratinocyte cell line. We investigated the atopic dermatitis (AD) effect of topically applied CIL in mice with AD-like symptoms. After topical application of 1,3-butylen glycol (control), CIL-Low (5%), CIL-High (30%), or 0.1% hydrocortisone (HC) on the AD-like skin lesions in DNCB-treated NC/Nga mice for 5 weeks, the ear thickness, mast cell infiltration, and serum immunoglobulin E (IgE), IgG1, IL-4 and interferon (IFN)-γ were measured. The gene expressions of IL-4, IL-13, and IFN-γ in the dorsal skin were assayed. CIL treatment dosedependently reduced severity of clinical symptoms of dorsal skin, ear thickness, and the number of mast cells and eosinophils. CIL-High significantly decreased serum IgE, IgG1, IL-4, and IFN-γ levels and reduced mRNA levels of IFN-γ, IL-4, and IL-13 in dorsal skin lesion. The improvement by CIL-High was similar to HC, but without its adverse effects such as skin atrophy maceration, and secondary infection. In conclusion, CIL may be an effective alternative substance for the management of AD.

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    ABSTRACT: Wild chrysanthemum (Chrysanthemum indicum L.) is traditionally used in folk medicine as an anti-inflammatory agent. It is also used in the southwest plateau region of China to prevent ultraviolet-induced skin damage. However, the role and mechanism by which wild chrysanthemum prevents UV-induced skin damage and photoaging have never been investigated in vitro. In the present study, we found that aqueous extracts from wild chrysanthemum strongly reduced high-dose UVB-induced acute cell death of human immortalized keratinocytic HaCat cells. Wild chrysanthemum extract was also demonstrated to reduce low-dose UVB-induced expression of the photoaging-related matrix metalloproteinases MMP-2 and MMP-9. The ROS level elevated by UVB irradiation was strongly attenuated by wild chrysanthemum extract. Further study revealed that wild chrysanthemum extract reduced UVB-triggered ERK1/2 and p38 MAPK phosphorylation and their protective role, which is partially dependent on inhibiting p38 activation. These results suggest that wild chrysanthemum extract can protect the skin from UVB-induced acute skin damage and photoaging by reducing the intracellular reactive oxygen species (ROS) level and inhibiting p38 MAPK phosphorylation. The present study confirmed the protective role of wild chrysanthemum against UV-induced skin disorders in vitro and indicated the possible mechanism. Further study to identify the active components in wild chrysanthemum extract would be useful for developing new drugs for preventing and treating skin diseases, including skin cancer and photoaging, induced by UV irradiation.
    Cytotechnology 07/2014; DOI:10.1007/s10616-014-9773-5 · 1.45 Impact Factor
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    ABSTRACT: Objectives We aimed to investigate the inhibitory efficacy of Solanum tuberosum L. var. Vitelotte (SV) extract on atopic dermatitis (AD)-like skin lesions induced by the topical application of 2,4-dinitrochlorobenzene in NC/Nga mice.MethodsSV extract was administered orally to NC/Nga mice at the dose of 75, 150 or 300 mg/kg for 4 weeks. The effectiveness of SV extract in NC/Nga mice was evaluated by measuring symptom severity, ear thickness, scratching behaviour, serum levels of IgE, IgG1 and IgG2a, T helper 1 (Th1; interferon-γ and IL-12) and Th2 cytokines (IL-4 and IL-13) in spleen, messenger RNA (mRNA) expression of inflammatory cytokines and chemokines in tissue and infiltration of inflammatory cells in tissue.Key findingsOral administration of SV extract to NC/Nga mice resulted in the inhibition of the development of AD-like skin lesions. SV extract was attenuated AD-like skin lesion, ear thickening and scratching behaviour. SV extract also alleviated infiltrated inflammatory cells in tissue. Production of Th1 and Th2 cytokines was inhibited in splenocyte cultures. Additionally, reduced levels of IgE and IgG1/IgG2a ratio in serum and expression of AD-related mRNAs in lesional skins were observed in SV-treated mice compared with control group.ConclusionsSV extract alleviated the exacerbation of AD-like skin lesions in NC/Nga mice by suppressing total serum level of IgE and correcting the Th1/Th2 balance.
    07/2014; DOI:10.1111/jphp.12254
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    ABSTRACT: Herbs are widely used in the treatment of atopic dermatitis (AD) in Eastern Asian countries, and certain herbs regarded have anti-inflammatory properties that can help with AD. With the goal of developing a topical herbal agent for AD, we conducted a systematic review of in vivo studies of AD-like skin models for screening potential herbs. Searches were conducted from PubMed and EMBASE. After all, 22 studies were included for this review. We judged most of the domains of all studies to be at unclear risk of bias. Among 22 included studies, 21 herbs have been reported to reduce AD-like skin lesions in mouse models by suppressing Th2 cell response. Our findings may offer potential herbs for the topical application treatment of AD.
    Mediators of Inflammation 06/2014; 2014:752103. DOI:10.1155/2014/752103 · 2.42 Impact Factor

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