Article

Zoonotic agents in small ruminants kept on city farms in southern Germany.

Department of Veterinary Sciences, Ludwig-Maximilians-Universitat Munich, Munich, Germany.
Applied and environmental microbiology (impact factor: 3.69). 03/2012; 78(11):3785-93. DOI:10.1128/AEM.07802-11 pp.3785-93
Source: PubMed

ABSTRACT Sheep and goats are popular examples of livestock kept on city farms. In these settings, close contacts between humans and animals frequently occur. Although it is widely accepted that small ruminants can carry numerous zoonotic agents, it is unknown which of these agents actually occur in sheep and goats on city farms in Germany. We sampled feces and nasal liquid of 48 animals (28 goats, 20 sheep) distributed in 7 city farms and on one activity playground in southern Germany. We found that 100% of the sampled sheep and 89.3% of the goats carried Shiga toxin-producing Escherichia coli (STEC). The presence of Staphylococcus spp. in 75% of both sheep and goats could be demonstrated. Campylobacter spp. were detected in 25% and 14.3% of the sheep and goats, respectively. Neither Salmonella spp. nor Coxiella burnetii was found. On the basis of these data, we propose a reasonable hygiene scheme to prevent transmission of zoonotic agents during city farm visits.

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  • Article: Development of a m-PCR assay for simultaneous identification of Campylobacter jejuni and C. coli.
    M Denis, C Soumet, K Rivoal, G Ermel, D Blivet, G Salvat, P Colin
    [show abstract] [hide abstract]
    ABSTRACT: Multiplex PCR assay (m-PCR) with three sets of primers was developed for simultaneous identification of Campylobacter jejuni and C. coli. Poultry faecal samples were enriched in Preston broth for 24 h and streaking on selective media was performed before and after enrichment. m-PCR was applied on bacterial cultures harvested from media plates. The data showed a selective effect of Preston broth which favoured the growth of C. coli. Identification of the species by the hippurate hydrolysis test and by the m-PCR was performed on 294 isolates of Campylobacter. The efficiency of the identification by the biochemical test is only 34% in comparison to 100% efficiency with the PCR. The use of our m-PCR in combination with the culture method allowed reliable detection and identification of C. jejuni and C. coli within 3-4 d.
    Letters in Applied Microbiology 01/2000; 29(6):406-10. · 1.62 Impact Factor
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Keywords

48 animals
 
7 city farms
 
activity playground
 
city farm visits
 
city farms
 
contacts
 
Germany
 
goats
 
humans
 
numerous zoonotic agents
 
reasonable hygiene scheme
 
Salmonella spp
 
sampled sheep
 
settings
 
Shiga toxin-producing Escherichia coli
 
small ruminants
 
southern Germany
 
STEC
 
zoonotic agents