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Ultrasonic-assisted extraction process of crude polysaccharides from Yunzhi mushroom and its effect on hydroxyproline and glycosaminoglycan levels

Department of Orthopaedics, Xinqiao Hospital, The Third Military Medical University, Chongqing 400037, China; Department of Anatomy, College of Basic Medicine, The Third Military Medical University, Chongqing 400038, China
Carbohydrate Polymers 01/2010; DOI: 10.1016/j.carbpol.2010.01.060

ABSTRACT Yunzhi is a mushroom belonging to species of the Basidiomycetes class of fungi. Its medicinal value was recorded in China. The Yunzhi polysaccharide was extracted from Yunzhi mushroom in this work. The results of chemical composition indicated that Yunzhi polysaccharides comprised mainly of carbohydrate compound. Total carbohydrate content was determined to be 95%. The polysaccharides were composed of glucose and mannose as detected by GC in the ratio of 4.4:1. The absorption spectra of the polysaccharides showed that the polysaccharides-related absorption peaks at 1153, 1336, 1460, 1663, 1712 and 2927 cm−1. Effect of Yunzhi polysaccharides on hydroxyproline, glycosaminoglycan, I collagens mRNA/β-actin and I collagens protein/GAPDH was investigated by employing animal model. Result showed that Yunzhi polysaccharides could enhance hydroxyproline, glycosaminoglycan, I collagens mRNA/β-actin and I collagens protein/GAPDH level in rat. These results indicated that Yunzhi polysaccharides could be beneficial for treatment of bone degenerative diseases.

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