Ultrasonic-assisted extraction process of crude polysaccharides from Yunzhi mushroom and its effect on hydroxyproline and glycosaminoglycan levels
Department of Anatomy, College of Basic Medicine, The Third Military Medical University, Chongqing 400038, China Carbohydrate Polymers
(Impact Factor: 4.07).
05/2010; 81(1):93-96. DOI: 10.1016/j.carbpol.2010.01.060
Yunzhi is a mushroom belonging to species of the Basidiomycetes class of fungi. Its medicinal value was recorded in China. The Yunzhi polysaccharide was extracted from Yunzhi mushroom in this work. The results of chemical composition indicated that Yunzhi polysaccharides comprised mainly of carbohydrate compound. Total carbohydrate content was determined to be 95%. The polysaccharides were composed of glucose and mannose as detected by GC in the ratio of 4.4:1. The absorption spectra of the polysaccharides showed that the polysaccharides-related absorption peaks at 1153, 1336, 1460, 1663, 1712 and 2927 cm−1. Effect of Yunzhi polysaccharides on hydroxyproline, glycosaminoglycan, I collagens mRNA/β-actin and I collagens protein/GAPDH was investigated by employing animal model. Result showed that Yunzhi polysaccharides could enhance hydroxyproline, glycosaminoglycan, I collagens mRNA/β-actin and I collagens protein/GAPDH level in rat. These results indicated that Yunzhi polysaccharides could be beneficial for treatment of bone degenerative diseases.
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- "Consequently, following initiation of the inflammation, macrophages secrete proinflammatory molecules such as IL-1, IL-6, and TNF-α . Also, collagen is a group of naturally occurring proteins, and it is the main protein of connective tissues making about 25-35% of the whole body protein content . Excessive production of collagen has been documented in proliferative disorders such as liver cirrhosis. "
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ABSTRACT: The technique of stem cells or hepatocytes transplantation has recently improved in order to bridge the time before whole-organ liver transplantation. In the present study, unfractionated bone marrow stem cells (BMSCs) were harvested from the tibial and femoral marrow compartments of male mice, which were cultured in Dulbecco's modified Eagle's medium (DMEM) with and without hepatocyte growth factor (HGF), and then transplanted into Schistosoma mansoni-infected female mice on their 8th week post-infection. Mice were sacrificed monthly until the third month of bone marrow transplantation, serum was collected, and albumin concentration, ALT, AST, and alkaline phosphatase (ALP) activities were assayed. On the other hand, immunohistopathological and immunohistochemical changes of granuloma size and number, collagen content, and cells expressing OV-6 were detected for identification of liver fibrosis. BMSCs were shown to differentiate into hepatocyte-like cells. Serum ALT, AST, and ALP were markedly reduced in the group of mice treated with BMSCs than in the untreated control group. Also, granuloma showed a marked decrease in size and number as compared to the BMSCs untreated group. Collagen content showed marked decrease after the third month of treatment with BMSCs. On the other hand, the expression of OV-6 increased detecting the presence of newly formed hepatocytes after BMSCs treatment. BMSCs with or without HGF infusion significantly enhanced hepatic regeneration in S. mansoni-induced fibrotic liver model and have pathologic and immunohistopathologic therapeutic effects. Also, this new therapeutic trend could generate new hepatocytes to improve the overall liver functions.
The Korean Journal of Parasitology 04/2014; 52(2):151-62. DOI:10.3347/kjp.2014.52.2.151 · 1.15 Impact Factor
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ABSTRACT: A high power ultrasound bath system has been used as a processing aid during sponge cake batter mixing in enhancing the mixing process to produce better quality of cake texture. The formulation for loading of 3 sponge cakes was mixed for 9min at 90rpm under different combinations of ultrasound power exposure ranging from 1 to 2.5kW, and for duration ranging from 3 to 9min. The ultrasound was able to enhance the mixing process by resulting in lower batter density and flow behavior index, higher overrun and viscosity compared to the non-aided mixing. With the 2.5kW ultrasound assisted mixing for entire batter mixing of 9min, a better cake quality was produced in terms of lower cake hardness, and higher cake springiness, cohesiveness and resilience. The aided ultrasound power and duration during cake batter mixing showed more significant effects on cake properties than its batter properties.
Journal of Food Engineering 06/2011; 104(3):430-437. DOI:10.1016/j.jfoodeng.2011.01.006 · 2.77 Impact Factor
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ABSTRACT: Objective: The polysaccharide from Kalimeris indica (KIP) has been proved to be one of effective parts with anti-gastric ulcer action. In order to accurately define the structure and structure-activity relationship of KIP, the purification and structural analysis of KIP were carried out. Methods: A water-soluble polysaccharide (KIP-1) was obtained from K. indica by hot water extraction, deproteination with enzymolysis and Sevag methods, and fractionation through DEAE cellulose (DE-52) column. The molecular weight of KIP-1 was determined by gel chromatography (Sephadex G-200). The structure was characterized with FTIR, GC, and GC-MS methylation analysis. The microstructure of KIP-1 was observed by atomic force microscope (AFM). Results: The results indicated that KIP-1 had a molecular weight of 11 592 and was mainly composed of mannose, glucose, and galactose with the ratio of 2.523:1.162:1. Methylation analysis showed that KIP-1 had a backbone of 1, 3-linked glucose and 1, 2-linked galactose, of which the branch point was at O-3 composed of a 1, 3, 6-linked mannose residue and terminated with mannose residue. The microstructure study showed that KIP-1 with an average particle size of 1-2 nm was granular, irregular-rounded, and ellipsoidal. Irregularly shaped structure was found together in cubic image of KIP-1 with the height about 1 nm. Conclusion: KIP-1 with branch structure is mainly composed of mannose, glucose, and galactose. It is the first time to get the polysaccharide from K. indica.
Chinese Traditional and Herbal Drugs 08/2013; 44(16):2213-2217. DOI:10.7501/j.issn.0253-2670.2013.16.004
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