Article

Ultrasonic-assisted extraction process of crude polysaccharides from Yunzhi mushroom and its effect on hydroxyproline and glycosaminoglycan levels

Department of Anatomy, College of Basic Medicine, The Third Military Medical University, Chongqing 400038, China
Carbohydrate Polymers (Impact Factor: 3.92). 05/2010; DOI: 10.1016/j.carbpol.2010.01.060

ABSTRACT Yunzhi is a mushroom belonging to species of the Basidiomycetes class of fungi. Its medicinal value was recorded in China. The Yunzhi polysaccharide was extracted from Yunzhi mushroom in this work. The results of chemical composition indicated that Yunzhi polysaccharides comprised mainly of carbohydrate compound. Total carbohydrate content was determined to be 95%. The polysaccharides were composed of glucose and mannose as detected by GC in the ratio of 4.4:1. The absorption spectra of the polysaccharides showed that the polysaccharides-related absorption peaks at 1153, 1336, 1460, 1663, 1712 and 2927 cm−1. Effect of Yunzhi polysaccharides on hydroxyproline, glycosaminoglycan, I collagens mRNA/β-actin and I collagens protein/GAPDH was investigated by employing animal model. Result showed that Yunzhi polysaccharides could enhance hydroxyproline, glycosaminoglycan, I collagens mRNA/β-actin and I collagens protein/GAPDH level in rat. These results indicated that Yunzhi polysaccharides could be beneficial for treatment of bone degenerative diseases.

0 Followers
 · 
65 Views
  • [Show abstract] [Hide abstract]
    ABSTRACT: The effects of ultrasonic power, extraction time, extraction temperature, and the water to raw material ratio on extraction yield of crude polysaccharide from the leaf of Hibiscus rosa-sinensis (HRLP) were optimized by statistical analysis using response surface methodology. The response surface methodology (RSM) was used to optimize HRLP extraction yield by implementing the Box-Behnken design (BBD). The experimental data obtained were fitted to a second-order polynomial equation using multiple regression analysis and also analyzed by appropriate statistical methods (ANOVA). Analysis of the results showed that the linear and quadratic terms of these four variables had significant effects. The optimal conditions for the highest extraction yield of HRLP were ultrasonic power: 93.59 w, extraction time: 25.71min, extraction temperature: 93.18°C, and the water to raw material ratio: 24.3mL/g. Under these conditions, the experimental yield was 9.66±0.18%, which is well in close agreement with the value predicted by the model9.526%. The results demonstrated that HRLP had strong scavenging activities in vitro on DPPH and hydroxyl radicals.
    International Journal of Biological Macromolecules 08/2014; DOI:10.1016/j.ijbiomac.2014.07.023 · 3.10 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: A Box–Behnken design was employed to optimize ultrasonic-assisted extraction of Trametes orientalis polysaccharides (TOP). The crude polysaccharides were purified by DEAE cellulose-52 chromatography, giving a main fraction named as PTOP. The antioxidant properties of PTOP were evaluated by different in vitro antioxidant assays, such as 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activities, reducing power, superoxide radical scavenging activities, and chelating ability of ferrous ions. The results showed that optimal extraction parameters were as follows: ratio of water to raw material 30.6 mL/g, ultrasonic power 109.8 W, extraction temperature 40.2 °C, and extraction time 42.2 min. Under these conditions, the experimental yield of polysaccharides was 7.49 ± 0.14%, which agreed closely with the predicted value (7.47%). Furthermore, PTOP exhibited antioxidant capacity in a concentration-dependent manner in all assays.
    Carbohydrate Polymers 10/2014; 111:315–323. DOI:10.1016/j.carbpol.2014.04.034 · 3.92 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: A Box-Behnken design (BBD) was used to evaluate the effects of ultrasonic power, extraction time, extraction temperature, and water to raw material ratio on extraction yield of alcohol-insoluble polysaccharide of Althaea rosea leaf (ARLP). Purification was carried out by dialysis method. Chemical analysis of ARLP revealed contained 12.69±0.48% moisture, 79.33±0.51% total sugar, 3.82±0.21% protein, 11.25±0.37% uronic acid and 3.77±0.15% ash. The response surface methodology (RSM) showed that the significant quadratic regression equation with high R(2) (=0.9997) was successfully fitted for extraction yield of ARLP as function of independent variables. The overall optimum region was found to be at the combined level of ultrasonic power 91.85w, extraction time 29.94min,extraction temperature 89.78°C, and the ratio of water to raw material 28.77(mL/g). At this optimum point, extraction yield of ARLP was 19.47±0.41%. No significant (p>0.05) difference was found between the actual and predicted (19.30±0.075%) values. The results demonstrated that ARLP had strong scavenging activities on DPPH and hydroxyl radicals.
    International Journal of Biological Macromolecules 08/2014; 72. DOI:10.1016/j.ijbiomac.2014.08.005 · 3.10 Impact Factor