Ultrasonic-assisted extraction process of crude polysaccharides from Yunzhi mushroom and its effect on hydroxyproline and glycosaminoglycan levels
ABSTRACT Yunzhi is a mushroom belonging to species of the Basidiomycetes class of fungi. Its medicinal value was recorded in China. The Yunzhi polysaccharide was extracted from Yunzhi mushroom in this work. The results of chemical composition indicated that Yunzhi polysaccharides comprised mainly of carbohydrate compound. Total carbohydrate content was determined to be 95%. The polysaccharides were composed of glucose and mannose as detected by GC in the ratio of 4.4:1. The absorption spectra of the polysaccharides showed that the polysaccharides-related absorption peaks at 1153, 1336, 1460, 1663, 1712 and 2927 cm−1. Effect of Yunzhi polysaccharides on hydroxyproline, glycosaminoglycan, I collagens mRNA/β-actin and I collagens protein/GAPDH was investigated by employing animal model. Result showed that Yunzhi polysaccharides could enhance hydroxyproline, glycosaminoglycan, I collagens mRNA/β-actin and I collagens protein/GAPDH level in rat. These results indicated that Yunzhi polysaccharides could be beneficial for treatment of bone degenerative diseases.
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ABSTRACT: Degree of substitution (DS) Pasting property Thermal property Granular morphology Confocal laser scanning microscopy (CLSM) High performance anion exchange chromatography (HPAEC) Response surface methodology (RSM) a b s t r a c t Granular cationic starches were prepared in aqueous phase without the addition of swelling inhibiting salts. Response surface methodology (RSM) was performed to analyze the effects of reaction conditions on physicochemical properties of the products. As the reaction time was prolonged from 2 to 5 and to 24 h, the relative contribution of the temperature to degree of substitution (DS) turned from minor to prominent. Good correlations were observed between the DS and the pasting temperature of the 2, 5, and 24 h cationized starches. By contrast, variation in the correlations between DS and the other physi-cochemical properties, with respect to reaction durations, revealed the processing pattern of cationiza-tion in starch granules along extending reaction times. This deduced pattern was confirmed by the granular and molecular characterizations using confocal laser scanning microscopy and high perfor-mance anion exchange chromatography, respectively.Carbohydrate Polymers - CARBOHYD POLYM. 10/2008; 75(4).
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ABSTRACT: An analog of proline, 2, 3-cis-3, 4-trans-3, 4-dihydroxy-L-proline, was found in the cell walls of the eight species of diatoms studied and was isolated from the proteinaceous material of the wall of Navicula pelliculosa. The properties of this substance are described; its structure was confirmed by nuclear magnetic resonance spectros-copy.Science 07/1969; 164(3886):1400-1. · 31.03 Impact Factor
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ABSTRACT: Exposure of adult rats to 0.8 ppm ozone enhanced collagen synthesis in the lungs. Collagen synthesis was studied by estimating hydroxyproline (Hyp) content and by following the activity of prolyl hydroxylase (PH), a crucial enzyme in the pathway of collagen biosynthesis. In the early phases (1–2 day) of ozone-induced injury, PH activity was increased twofold over control values and the amount of collagen synthesized (as estimated by Hyp formation) was double the amount of non-collagenous protein synthesized. This resulted, by the third day, in a significant increase (29%) in total lung collagen. In the later stages of the injury (3–7 day), however, increases in PH activity were more gradual, approaching 2.7 times control levels at the end of the 7-day exposure period. The synthesis of non-collagenous protein during this period increased steadily and by the 7th day the ratio of the amounts of collagen to non-collagenous protein synthesized was comparable to that of controls. When the exposed (0.8 ppm O3/7 days) animals were placed in filtered ambient air, PH activity returned to normal in 13 days whereas Hyp content remained elevated for up to 28 days. These results suggest that environmental ozone exposure could be a contributing factor in pulmonary disorders involving lung collagen synthesis.Life Sciences 06/1976; 18(9):897-903. · 2.56 Impact Factor