Ultrasonic-assisted extraction process of crude polysaccharides from Yunzhi mushroom and its effect on hydroxyproline and glycosaminoglycan levels
ABSTRACT Yunzhi is a mushroom belonging to species of the Basidiomycetes class of fungi. Its medicinal value was recorded in China. The Yunzhi polysaccharide was extracted from Yunzhi mushroom in this work. The results of chemical composition indicated that Yunzhi polysaccharides comprised mainly of carbohydrate compound. Total carbohydrate content was determined to be 95%. The polysaccharides were composed of glucose and mannose as detected by GC in the ratio of 4.4:1. The absorption spectra of the polysaccharides showed that the polysaccharides-related absorption peaks at 1153, 1336, 1460, 1663, 1712 and 2927 cm−1. Effect of Yunzhi polysaccharides on hydroxyproline, glycosaminoglycan, I collagens mRNA/β-actin and I collagens protein/GAPDH was investigated by employing animal model. Result showed that Yunzhi polysaccharides could enhance hydroxyproline, glycosaminoglycan, I collagens mRNA/β-actin and I collagens protein/GAPDH level in rat. These results indicated that Yunzhi polysaccharides could be beneficial for treatment of bone degenerative diseases.
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ABSTRACT: Ziziphus jujuba Mill. is nutritious and used as food and medicine for more than two thousand years. It has many pharmacological effects, such as elimination of fatigue, dilation of blood vessels, etc. The polysaccharide in it is one of the bioactive substances. In this paper, the ultrasonic extraction effects on the yield and activity of polysaccharide were studied. The optimum ultrasonic extraction conditions were investigated based on a Box-Behnken statistical experimental design. Response surface methodology (RSM) of three factors (ultrasonic power, extraction time and extraction temperature) and three levels was employed to optimize the yield and the antioxidant activity of the polysaccharides. The experimental data were fitted to quadratic response surface models using multiple regression analysis. The best extraction conditions were 120 W, 15 min. and 55[degree sign]C for highest yield, and 80 W, 15 min. and 40[degree sign]C for highest hydroxyl radical scavenging activity. The study showed that high ultrasonic power was good for obtaining high yield but bad for keeping the antioxidant activity of the polysaccharides.Chemistry Central Journal 09/2013; 7(1):160. · 1.31 Impact Factor