Hydrolysis of wood saw dust by combined chemical pretreatment and enzymatic methods for lignocellulosic saccharification

GERF Bulletin of Biosciences 12/2011; 2(2):29-31.

ABSTRACT Wood saw dust (WSD) after lignocellulosic saccharification by different hydrolysis methods is more efficient for ethanol production as, its contains cellulose and hemi-cellulose at higher levels 65% (w/v) and 35% (w/v), respectively. Cellulose and hemicellulose account for about a quarter of whole biomass in all land plants. A pretreatment method using chemical hydrolysis and enzymatic conversion from starch into fermentable sugars was investigated. The WSD was hydrolyzed at 1.69 g/l, using a crude culture filtrate Aspergillus fumigatus at pH 5.0 and 30ºC in acetate buffer 50 mM, while 23.3 g/ l was with 1 N sulfuric acid (H2SO4) treatment. Aonla pomace waste was used as substitute to acid because of high acidic nature. Optimum conditions for lignocellulosic saccharification is discussed in this paper.

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