Extraction, isolation and characterization of bioactive compounds from plants' extracts.

Institute for Research in Molecular Medicine, Universiti Sains Malaysia, Minden, Malaysia.
African Journal of Traditional, Complementary and Alternative Medicines (Impact Factor: 0.56). 01/2011; 8(1):1-10. DOI: 10.4314/ajtcam.v8i1.60483
Source: PubMed

ABSTRACT Natural products from medicinal plants, either as pure compounds or as standardized extracts, provide unlimited opportunities for new drug leads because of the unmatched availability of chemical diversity. Due to an increasing demand for chemical diversity in screening programs, seeking therapeutic drugs from natural products, interest particularly in edible plants has grown throughout the world. Botanicals and herbal preparations for medicinal usage contain various types of bioactive compounds. The focus of this paper is on the analytical methodologies, which include the extraction, isolation and characterization of active ingredients in botanicals and herbal preparations. The common problems and key challenges in the extraction, isolation and characterization of active ingredients in botanicals and herbal preparations are discussed. As extraction is the most important step in the analysis of constituents present in botanicals and herbal preparations, the strengths and weaknesses of different extraction techniques are discussed. The analysis of bioactive compounds present in the plant extracts involving the applications of common phytochemical screening assays, chromatographic techniques such as HPLC and, TLC as well as non-chromatographic techniques such as immunoassay and Fourier Transform Infra Red (FTIR) are discussed.

  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Rotenone is one of the prominent insecticidal isoflavonoid compounds which can be isolated from the extract of Derris sp. plant. Despite being an effective compound in exterminating pests in a minute concentration, procuring a significant amount of rotenone in the extracts for commercialized biopesticides purposes is a challenge to be attained. Therefore, the objective of this study was to determine the best ionic liquid (IL) which gives the highest yield of rotenone. The normal soaking extraction (NSE) method was carried out for 24 hrs using five different types of binary solvent systems comprising a combination of acetone and five respective ionic liquids (ILs) of (1) [BMIM] Cl; (2) [BMIM] OAc; (3) [BMIM] NTf2; (4) [BMIM] OTf; and (5) [BMPy] Cl. Next, the yield of rotenone, % (w/w), and its concentration (mg/mL) in dried roots were quantitatively determined by means of RP-HPLC and TLC. The results showed that a binary solvent system of [BMIM] OTf + acetone was the best solvent system combination as compared to other solvent systems (p < 0.05). It contributed to the highest rotenone content of 2.69 ± 0.21% (w/w) (4.04 ± 0.34 mg/mL) at 14 hrs of exhaustive extraction time. In conclusion, a combination of the ILs with a selective organic solvent has been proven to increase a significant amount of bioactive constituents in the phytochemical extraction process.
    E-Journal of Chemistry 02/2015; 2015(Article ID 468917):7. · 0.62 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: The enzyme 3-hydroxy-3-methyl-glutaryl-coenzyme A (HMG-CoA) reductase is the key enzyme of the mevalonate pathway that produces cholesterol. Inhibition of HMG-CoA reductase reduces cholesterol biosynthesis in the liver. Synthetic drugs, statins, are commonly used for the treatment of hypercholesterolemia. Due to the side effects of statins, natural HMG-CoA reductase inhibitors of plant origin are needed. In this study, 25 medicinal plant methanol extracts were screened for anti-HMG-CoA reductase activity. Basella alba leaf extract showed the highest inhibitory effect at about 74%. Thus, B. alba was examined in order to investigate its phytochemical components. Gas chromatography with tandem mass spectrometry and reversed phase high-performance liquid chromatography analysis revealed the presence of phenol 2,6-bis(1,1-dimethylethyl), 1-heptatriacotanol, oleic acid, eicosyl ester, naringin, apigenin, luteolin, ascorbic acid, and α-tocopherol, which have been reported to possess antihypercholesterolemic effects. Further investigation of in vivo models should be performed in order to confirm its potential as an alternative treatment for hypercholesterolemia and related cardiovascular diseases.
    Drug Design, Development and Therapy 01/2015; 9:509-17. · 3.03 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Objective: Teucrium polium L. aerial parts have been used by local populations of Palestine for the treatment of various diseases as diabetes mellitus, amoeba, intestinal spasms and diarrhea for that the interest of using this herbal remedy among researchers and populations increased, this study was aimed to evaluate the best area in Palestine that may be considered the best source of collection and cultivation for Teucrium polium plant in the future, to be a good source for manufacturing active pharmaceutical dosage forms from this plant. Method: The yield of the organic and water extraction method assessed by using exhaustive extraction method, by steeping the plant in ethanol, water and hexane to extract all the chemical ingredients without heating to prevent destroying or changing the plant chemical constituents. Results: the best aqueous and organic yields were in Bethlehem region (6.4%, 2.6 %), while the lowest aqueous and organic yields were in Qalqilya (4.96%, 1.16%). Conclusion: We recommended the pharmaceutical companies; Bethlehem region as the best area for cultivation and collection Teucrium plant for manufacturing the best standardized pharmaceutical dosage forms also we recommended the researchers to use the Teucrium plant from this region for their scientific research.
    International Journal of Pharmacy and Pharmaceutical Sciences 01/2015; 7(1). · 1.59 Impact Factor

Full-text (2 Sources)

Available from
Jun 16, 2014