Extraction, Isolation and Characterization of Bioactive Compounds from Plants’ Extracts

Institute for Research in Molecular Medicine, Universiti Sains Malaysia, Minden, Malaysia.
African Journal of Traditional, Complementary and Alternative Medicines (Impact Factor: 0.56). 01/2011; 8(1):1-10. DOI: 10.4314/ajtcam.v8i1.60483
Source: PubMed


Natural products from medicinal plants, either as pure compounds or as standardized extracts, provide unlimited opportunities for new drug leads because of the unmatched availability of chemical diversity. Due to an increasing demand for chemical diversity in screening programs, seeking therapeutic drugs from natural products, interest particularly in edible plants has grown throughout the world. Botanicals and herbal preparations for medicinal usage contain various types of bioactive compounds. The focus of this paper is on the analytical methodologies, which include the extraction, isolation and characterization of active ingredients in botanicals and herbal preparations. The common problems and key challenges in the extraction, isolation and characterization of active ingredients in botanicals and herbal preparations are discussed. As extraction is the most important step in the analysis of constituents present in botanicals and herbal preparations, the strengths and weaknesses of different extraction techniques are discussed. The analysis of bioactive compounds present in the plant extracts involving the applications of common phytochemical screening assays, chromatographic techniques such as HPLC and, TLC as well as non-chromatographic techniques such as immunoassay and Fourier Transform Infra Red (FTIR) are discussed.

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Available from: Yeng Chen, Jun 11, 2014
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    • "Herbs are also included in the bitter extracts preparation. Their original pharmaceutical use stimulated also the making of bitter extracts to be used to prepare liquors and elixirs with beneficial effects on human [3] [4] [5] The complexity of the vegetal matrix to extract, often made from a mixture of different parts from several plants causes difficulty to reproduce the same extract due to the natural variability of the constituents of each plant from one year to another. "
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    DESCRIPTION: Bitter liquors and elixirs are usually produced following traditional recipes, in most cases considered secret, that adopts different extraction processes and/or way of preparation. The ethanol as elective extraction solvent allows to obtain in the liquid phase most of the active compounds from different parts of the plants e.g. roots, bark, flowers, etc and the maceration is commonly used as extraction process. In this paper the traditional maceration procedure was compared to an innovative technology of rapid solid-liquid dynamic extraction (RSLDE), using the Naviglio Extractor. to obtain alcoholic extract of herbal mixtures.Three different mixtures of various parts of plants were extracted with both methods and results compared. The dry residue was considered as the main parameters to assess the quantity of the active compounds present in the extracts. The obtained data suggested that an high dry residue amount in the extract was correlated to an high concentration of active ingredients. Organoleptic tests performed on bitter liquors obtained from different extracts has been used to determine the optimum extraction time for the two different methods used. The results of this test showed that the bitters produced with RLSDE were more appreciated than bitter prepared by maceration.
    • "Medicinal plants provide a wide range of active agents for a variety of pathologies [1] [2]. As reported by the World Health Organization (WHO), 60% to 80% of the population call for natural medicine in the case of its primary healthcare needs [3].The isoflavone genistein (4',5,7-trihydroxyisoflavone) is the aglycone of the heteroside genistin. It can be found mainly in plants of the Fabaceae family being best represented in soybean (Glycine max L. Merr.) seeds [4]. "
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    ABSTRACT: In recent years polyurethane microstructures (PM) have gained increasing attention in the pharmaceutical field due to the importance of their practical application. Since finding that such a formulation with genistein could improve its applications, we have conducted a preliminary study regarding the in vitro antiproliferative (MCF7, MDA-MB-231 and T47D) and antimicrobial (Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Salmonella enteritidis (D), Bacillus subtilis, B. cereus, and Candida albicans) activity in order to test whether polyurethane micro structuresre present a good option for further modulation of genistein's bioavailability. It was concluded that the polyurethane micro structures are a bad in vitro partner for the isoflavone genistein.
    Natural product communications 06/2015; 10(6):951-4. · 0.91 Impact Factor
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    • "For thousands of years natural products from medicinal plants and other sources are known to be used extensively as remedies for human health (Thitiya, 2000). This is due to the presence of active chemical compounds/constituents which produce definite physiological action on human body (Borris, 1996; Thitiya, 2000; Sasidharan et al., 2011). WHO encourages the use traditional medicines in health care programs in developing countries because of the great potential they hold in prevention of various diseases (Amos et al., 2001). "
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    ABSTRACT: The methanol stem bark extract of Neocarya macrophylla was screened for its analgesic activity using acetic acid-induced writhing in mice and formalin-induced pain in rats. The results of the study showed that the extract (60mg/kg, i.p.) decreased writhing response with 63.9% inhibition. The methanol extract also exhibited significant analgesic effect (P<0.05) in the formalin test which is in the same order of magnitude as that observed after administration of pentazocine (10mg/kg, i.p.) the standard drug. The intraperitoneal median lethal dose (LD50) of the methanol extract of N. macrophylla was found to be 283mg/kg in mice suggesting the plant is fairly toxic. The results of the study have shown that the methanol extract of N. macrophylla possesses analgesic activity which rationalizes the traditional use of the plant in the management of pain.
    Journal of Applied Pharmaceutical Science 04/2015; 5(Suppl 1):61-64. DOI:10.7324/JAPS.2015.54.S10 · 0.47 Impact Factor
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