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Is Acupuncture Meridians a Novel System for Superoxide Disposition

In book: Acupuncture - Concepts and Physiology
Source: InTech
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    ABSTRACT: To investigate the reactive oxygen species (ROS) distribution in living animal tissues, two ROS indicators, dichlorofluorescin diacetate (DCFH-DA) and MitoSOXä Red were applied to visualize ROS on the frontal interior abdominal wall of living SD-rats by tail vein injection and local smearing respectively. Revealed was an unexpected ROS distribution pattern. ROS were demonstrated in a few vertical fluorescent lines, which related to neither veins nor nerves but could be almost perfectly superimposable on a standard human acupunc-ture meridian network. The phenomenon that cells with high ROS content should be aligned in a regular manner is interesting as well as its resemblance to meridian system. Reactive oxygen species (ROS) are a variety of molecular oxygen-derived molecules and free radicals playing impor-tant roles from cell signaling to oxidative stress and cellular damages [1]. As an efficient approach in intracellular ROS studies, various ROS indicators have been developed. While dichlorofluorescin diacetate (DCFH-DA) emits green fluo-rescent light upon interaction with ROS after being hydro-lyzed into 2 0 ,7 0 -dichlorofluorescin(DCFH) by mitochondrial esterase [2], MitoSOX Red emits red fluorescent light when oxidized by mitochondrial superoxide [3]. In an attempt to investigate the ROS distribution in living animal tissues, those two indicators were employed to visualize ROS on the frontal interior abdominal wall of living SD-rats. An unex-pected pattern of ROS distribution was revealed, on which a standard human acupuncture meridian lines could be almost perfectly superimposed. Male SD rats of 6 weeks were used. 1 ml of DCFH-DA solution (10 mg in 1 ml dimethyl sulfoxide) was injected through the rat tail vein. 30 minutes later, the rat frontal abdominal wall was incised from over iliac crest then along the midaxillary line on both sides up to the rib bone so that the interior wall could be exposed for fluorescent imaging. On the other hand, the interior abdominal wall thus exposed was applied with MitoSOX Red reagent (100 mg in 1 ml dimethyl sulfoxide) by direct smearing with living rats without any other prior treatments. As is demonstrated in Fig. 1, fluorescent imaging was carried out with the exciting lights, which were generated by an UltraFire MCU WF-1200L lamp through band-pass filters of 488 nm for DCFH-DA and 510 nm for MitoSOX Red reagent, respectively. The fluorescence images of the
    Bioscience Hypotheses 07/2009; 2:443-445. DOI:10.1016/j.bihy.2009.07.005
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    ABSTRACT: This study aimed to understand energy metabolism distribution along the pericardium meridian and verify the correlation between the body surface (points), and classic meridian theory. A highly sensitive CO(2) instrument was used to measure the transcutaneous CO(2) emission at 13 points along the pericardium meridian line (12 points on the line and one point beyond the line) and 13 control points beside them. Results showed that the distribution of transcutaneous CO(2) emission is highly related to the position on the body. Transcutaneous CO(2) emission is significantly higher at P7 and P3, than the control points beside them. The points along the meridian and the points beside them were clustered with relative distance by SAS statistics software. Two distance matrixes were then obtained. The correlation coefficients between the points along the line and between the control points were calculated. The results showed that the 13(th) point beyond the line was far from the 12 points on the line (distance, 0.24), while acupoints on the line clustered earlier when compared with the non-acupoints. The average correlation coefficients among the acu-points was 0.65 which was significantly higher than 0.56, among the non-acupoints. No such characteristics were found among the control points. It was concluded that there is a strong correlativity of energy metabolism activity between the body surfaces along the meridian, and an even stronger correlativity between the acupoints on the meridian.
    09/2009; 2(3):197-201. DOI:10.1016/S2005-2901(09)60055-8
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    ABSTRACT: Obesity is a worldwide public health issue, and the prevalence of obesity is also increasing steadily in Taiwan. Obesity leads to several chronic diseases. Often, impaired quality of life is a consequence of obesity. The aim of this study was to determine if body weight-loss could improve health-related quality of life (HRQOL) in Taiwan. The cutoff for obesity is body mass index (BMI) = 27 as compared with 30 in US and Europe. We enrolled 67 participants with BMI ≥ 27 and more than one criterion of the metabolic syndrome, who underwent 3 months of body weight-loss intervention by diet control and regular exercise. We performed anthropometric measurements and blood tests, and administered the WHOQOL-BREF questionnaire Taiwanese version to assess HRQOL before and after the weight loss intervention. This is the first study using the WHOQOL-BREF to examine HRQOL in Taiwan. The questionnaire included D1 physical, D2 psychological, D3 social relationships and D4 environmental domains; each was scored from 4 to 20. Obese subjects had lower D1 and D2 scores as compared with the Taiwan healthy population reference group at baseline. In this study, 38 participants completed the 3-month intervention program and 29 participants dropped out. Twenty-five participants reached the 5% of initial BMI goal. Among them, significant statistical improvements were found both in medical comorbidities and in the four domains of the HRQOL questionnaire. Obesity can cause impaired HRQOL, which can be improved through BMI intervention. In addition to the benefits of biomedical aspect, this could be an incentive goal for keeping body weight control.
    Journal of the Chinese Medical Association 04/2011; 74(4):169-75. DOI:10.1016/j.jcma.2011.01.038 · 0.75 Impact Factor

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