Antihyperglycemic and antioxidant effect of hydroethanolic extract of Butea monosperma bark in diabetic mice.
ABSTRACT The antihyperglycemic, antihyperlipidemic and antioxidative properties of hydroethanolic extract of Butea monosperma bark were investigated in alloxan-induced diabetic mice. Alloxan administration resulted in higher blood glucose level and reduced hepatic glycogen content as compared to normal animals. Besides, serum lipid profile parameters such as total cholesterol (TC), triglyceride (TG), low density lipoprotein (LDL) and very low density lipoprotein (VLDL) cholesterol were also found to be significantly elevated, whereas the level of high density lipoprotein (HDL) cholesterol was markedly reduced in diabetic animals. Oxidative damage in the tissues of diabetic mice was evidenced by a marked increase in the level of thiobarbituric acid reactive substances (TBARS), distinct decrease in reduced glutathione (GSH) content and declined activity of antioxidant enzymes, such as superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px). The daily treatment of diabetic animals with crude extract of B. monosperma bark (300 mg kg(-1)) for 45 days significantly lowered blood glucose level and elevated hepatic glycogen content, bringing the values close to those observed in normal control and glibenclamide-treated diabetic mice. Furthermore, the level of various lipid profile parameters was also reversed towards normal. TBARS and GSH also restored towards normal and the declined activity of antioxidant enzymes in diabetic animals was also normalized in crude extract administered mice, thus indicating the antioxidant efficacy of the drug in diabetes-induced oxidative damage. Significant antihyperglycemic and antioxidant potential of the crude extract of B. monosperma bark indicated that it may find use in the management of diabetes and resultant oxidative stress.
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- "Taub. bark in diabetic mice (Sharma and Garg, 2012). The hypoglycemic effects of an aqueous extract of Bauhinia forficata Link on the salivary glands of diabetic mice has been shown (Curcio et al., 2012). "
ABSTRACT: Antihyperglycemic and antinociceptive activity of Fabaceae family plants – an evaluation of Mimosa pigra L. leaves ABSTRACT The objective of the present study was to evaluate the antihyperglycemic and antinociceptive activity of methanolic extract of leaves of Mimosa pigra, a plant used by folk medicinal practitioners of Bangladesh for lowering of blood sugar in diabetic patients and for the treatment of pain. Antihyperglycemic activity tests were conducted in glucose-loaded Swiss albino mice. The extract at doses of 50, 100, 200 and 400 mg per kg body weight mice was observed to significantly and dose-dependently reduce the concentration of blood glucose levels in glucose-loaded mice. At the above four doses, the extract, respectively, lowered blood glucose levels by 32.75, 35.16, 47.59 and 56.82%. A standard antihyperglycemic drug, glibenclamide, when administered at a dose of 10 mg per kg body weight in mice, lowered blood glucose levels by 56.82% thus showing that the highest dose of the extract had equivalent potency to that of glibenclamide in reducing blood glucose levels, and so merits further potential for isolation and identification of responsible phytochemical constituents, which can possibly be used as novel antihyperglycemic drugs. Antinociceptive activity tests were done in gastric pain model mice where gastric pain was induced by intraperitoneal administration of acetic acid, and subsequently the number of gastric pain-induced writhings was counted. The extract was also observed to significantly and dose-dependently reduce the number of gastric writhings. At doses of 50, 100, 200 and 400 mg per kg body weight, the extract reduced the number of writhings, respectively, by 27.27, 33.27, 51.45 and 54.55%. In comparison, a standard antinociceptive drug, aspirin, reduced the number of writhings, respectively, by 27.27 and 36.36% when administered to mice. Thus dose for dose, the extract was more potent than aspirin in reducing pain. The results not only validates the folk medicinal use of this plant in reducing blood sugar and for alleviation of pain, but also suggests that the plant can be further explored for discovery of possible antihyperglycemic and pain-killing drugs.
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ABSTRACT: The main objective of the study was to evaluate the antihyperglycemic, antidyslipidymic and antioxidative properties of methanolic leaf extract of Stevia rebaudiana in alloxan induced diabetic mice. Four groups of mice were studied. Group NC consisted of control animals. Rest three were made diabetic with a single intraperitoneal injection of alloxan monohydrate. Group DR was treated for 21 days with leaf extract of S.rebaudiana. After completion of experimental duration mice were sacrificed and blood and organs were further used for detecting biochemical and histopathological changes. Alloxan administration resulted in higher blood glucose level as compared to normal animals. Further, serum lipid profile parameters such as total cholesterol (TC), triglyceride (TG), low density lipoprotein (LDL) and very low density lipoprotein (VLDL) were also found to be significantly elevated, whereas high density lipoprotein (HDL) was reduced in diabetic animals. Oxidative damage in the tissues of diabetic animals shows a marked increase in the level of thiobarbituric acid reactive substances and reduced glutathione (GSH); and decrease in the activity of antioxidant enzymes such as Superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px). The daily treatment of methanolic extract of Stevia rebaudiana leaves at 300mg/kg for 21 days significantly lowered the blood glucose level and elevated hepatic glycogen content. Moreover, the levels of various lipid profile parameters also reversed towards normal. TBARS and GSH were also restored towards normal. Significant antihyperglycemic and antioxidant potential of the Stevia rebaudiana extract indicated that it may be used in the management of diabetes and resultant oxidative stress.International Journal of Pharmacy and Pharmaceutical Sciences 01/2013; 5(2):297-302. · 1.59 Impact Factor
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ABSTRACT: Abstract Background: The goal of the study was to determine the antidiabetic mechanisms and the antioxidant effects of aqueous (decoction and maceration) and methanol extracts from the stem bark of Ceiba pentandra. Methods: These extracts were tested in vitro on glucose uptake by skeletal muscles and liver slices and on glucose release by liver slices. The antioxidant activities of C. pentandra extracts were investigated at concentrations ranging from 1 to 300 µg/mL on 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydrogen peroxide (H2O2)-induced hemolysis, H2O2-induced brain lipid peroxidation, hydroxyl (˙OH) radical as well as their reducing power. Results: The decoction similarly to insulin exhibited a significant glucose lowering activity. In a hyperglycemic milieu, it significantly increased glucose uptake by the liver by 56.57% and in the skeletal muscle by 94.19%. In a hypoglycemic milieu, it significantly reduced glucose release by the liver by 33.94%. The decoction, maceration and methanol extracts exhibited a significant radical scavenging activity on DPPH with respective EC50 of 87.84, 54.77 and 6.15 µg/mL versus 2.24 µg/mL observed with ascorbic acid. All the extracts showed a significant antioxidant effect on hydroxyl radical, against lipid peroxidation and H2O2-induced hemolysis. The decoction showed the greatest antihemolytic effect with a maximum inhibition of 77.57% at the concentration of 100 µg/mL. C. pentandra extracts also showed a concentration-dependent reducing power. Conclusions: These results suggest that the antidiabetic effect of C. pentandra is due to its ability to increase glucose uptake and to reduce glucose release by target organs. The antioxidant properties of C. pentandra extracts are additional benefit for their antidiabetic effects.Journal of Complementary and Integrative Medicine 09/2014; 11(3):185-193. DOI:10.1515/jcim-2014-0031