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Antihyperglycemic and antioxidant effect of hydroethanolic extract of Butea monosperma bark in diabetic mice.

Department of Bioscience and Biotechnology, Banasthali University, Banasthali, Rajasthan-304022, India.
Indian journal of biochemistry & biophysics (Impact Factor: 1.03). 02/2012; 49(1):55-62.
Source: PubMed

ABSTRACT The antihyperglycemic, antihyperlipidemic and antioxidative properties of hydroethanolic extract of Butea monosperma bark were investigated in alloxan-induced diabetic mice. Alloxan administration resulted in higher blood glucose level and reduced hepatic glycogen content as compared to normal animals. Besides, serum lipid profile parameters such as total cholesterol (TC), triglyceride (TG), low density lipoprotein (LDL) and very low density lipoprotein (VLDL) cholesterol were also found to be significantly elevated, whereas the level of high density lipoprotein (HDL) cholesterol was markedly reduced in diabetic animals. Oxidative damage in the tissues of diabetic mice was evidenced by a marked increase in the level of thiobarbituric acid reactive substances (TBARS), distinct decrease in reduced glutathione (GSH) content and declined activity of antioxidant enzymes, such as superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px). The daily treatment of diabetic animals with crude extract of B. monosperma bark (300 mg kg(-1)) for 45 days significantly lowered blood glucose level and elevated hepatic glycogen content, bringing the values close to those observed in normal control and glibenclamide-treated diabetic mice. Furthermore, the level of various lipid profile parameters was also reversed towards normal. TBARS and GSH also restored towards normal and the declined activity of antioxidant enzymes in diabetic animals was also normalized in crude extract administered mice, thus indicating the antioxidant efficacy of the drug in diabetes-induced oxidative damage. Significant antihyperglycemic and antioxidant potential of the crude extract of B. monosperma bark indicated that it may find use in the management of diabetes and resultant oxidative stress.

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    ABSTRACT: The main objective of the study was to evaluate the antihyperglycemic, antidyslipidymic and antioxidative properties of methanolic leaf extract of Stevia rebaudiana in alloxan induced diabetic mice. Four groups of mice were studied. Group NC consisted of control animals. Rest three were made diabetic with a single intraperitoneal injection of alloxan monohydrate. Group DR was treated for 21 days with leaf extract of S.rebaudiana. After completion of experimental duration mice were sacrificed and blood and organs were further used for detecting biochemical and histopathological changes. Alloxan administration resulted in higher blood glucose level as compared to normal animals. Further, serum lipid profile parameters such as total cholesterol (TC), triglyceride (TG), low density lipoprotein (LDL) and very low density lipoprotein (VLDL) were also found to be significantly elevated, whereas high density lipoprotein (HDL) was reduced in diabetic animals. Oxidative damage in the tissues of diabetic animals shows a marked increase in the level of thiobarbituric acid reactive substances and reduced glutathione (GSH); and decrease in the activity of antioxidant enzymes such as Superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px). The daily treatment of methanolic extract of Stevia rebaudiana leaves at 300mg/kg for 21 days significantly lowered the blood glucose level and elevated hepatic glycogen content. Moreover, the levels of various lipid profile parameters also reversed towards normal. TBARS and GSH were also restored towards normal. Significant antihyperglycemic and antioxidant potential of the Stevia rebaudiana extract indicated that it may be used in the management of diabetes and resultant oxidative stress.
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