Article

[The morphological and karyological characteristics of MDCK and vero (B) cells cultures on plant hydrolyzate-based nutrient media].

Voprosy virusologii 56(2):9-14. pp.9-14
Source: PubMed

ABSTRACT MDCK and Vero (B) cell cultures were propagated during 10 passages in the experimental nutrient media containing the soybean powder hydrolyzate prepared using trypsin and bromelain enzymes and the rice powder hydrolysate prepared with trypsin and in the control DMEM and SFM4 MegaVir media. The karyological, morphological, and proliferative characteristics of continuous cultures were examined and compared. The experimental media supplied with 3% fetal bovine serum (FBS) (Gibco, U.S.A.) showed high growth-enhancing properties and failed to affect their morphology. After propagated during 10 passages in the experimental media preserved a stable karyotype. MDCK cell cultures in the nutrient media based on rice and soybean powder hydrolyzates low (2%) in FBS caused no substantial changes in the proliferation indices and morphological and karyological characteristics of cell cultures.

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Keywords

3% fetal bovine serum
 
bromelain enzymes
 
continuous cultures
 
control DMEM
 
experimental media
 
experimental nutrient media
 
FBS
 
growth-enhancing properties
 
karyological
 
karyological characteristics
 
MDCK
 
MDCK cell cultures
 
nutrient media
 
proliferation indices
 
proliferative characteristics
 
rice powder hydrolysate
 
soybean powder hydrolyzate
 
soybean powder hydrolyzates low
 
stable karyotype